血清内脂素作为人体衰老生物学标志物的相关性研究

Correlation Study of Serum Visfatin as Human Aging Biomarker

目的 探讨血清内脂素水平作为评估人体衰老程度的生物学标志物的潜力。方法 共招募248例年龄25~90岁的受试者,采用酶联免疫吸附试验测定血清内脂素水平,结合各项身体指标进行统计学分析。结果 Spearman相关性分析结果显示,血清内脂素水平与年龄呈负相关(r=-0.186,P=0.003)。将受试者按年龄分为3个区间,采用方差分析或秩和检验结果显示,年龄与血清内脂素水平、估算肾小球滤过率、胰岛素样生长因子1、免疫球蛋白M呈负相关;与体重指数、腰臀比、全身脂肪质量、全身脂肪质量指数、D-3羟基丁酸、游离脂肪酸、空腹血糖、胰岛素、胰岛素抵抗指数、胆碱酯酶、乳酸脱氢酶、谷丙转氨酶、谷草转氨酶、谷氨酰转肽酶、血清肌酐、血尿素氮、胱抑素C、血清同型半胱氨酸呈正相关。将受试者按血清内脂素水平分为3个区间,采用方差分析或秩和检验结果显示,血清内脂素水平与血清同型半胱氨酸(P=0.001)呈负相关,与IgM (P=0.013)呈正相关。结论 血清内脂素水平随年龄增加而降低,是一种潜在的衰老生物学标志物。

Objective To explore the potential of serum visfatin level as a biomarker for evaluating the degree of human aging.Methods A total of 248 participants aged 25-90 years were recruited,and the serum visfatin levels were detected by enzyme linked immunosorbent assay,and statistical analysis was performed combined with physical and biochemical indexes.Results The results of Spearman correlation analysis showed that serum visfatin levels were negatively correlated with age(r=-0.186,P=0.003).The participants were divided into three groups according to age.The results of ANOVA and rank-sum test showed that age was negatively correlated with serum visfatin levels,estimated glomerular filtration rate,insulin-like growth factor-1,IgM;positively correlated with body mass index,waist-to-hip ratio,body fat mass,body fat mass index,D-3hydroxybutyrate,free fatty acid,fasting blood glucose,insulin,HOMA-IR,cholinesterase,lactate dehydrogenase,alanine aminotransferase,aspartate aminotransferase,glutamyl transpeptidase,serum creatinine,blood urea nitrogen,cystatin C and serum homocysteine.The participants were divided into three groups according to serum visfatin levels.The results of ANOVA and rank-sum test showed that serum visfatin levels were negatively correlated with serum homocysteine(P=0.001),positively correlated with IgM(P=0.013).Conclusion Serum visfatin levels decrease with age,which is a potential aging biomarker.