乙型肝炎相关性肝硬化患者血清circMTO1表达水平及其与肝纤维化程度、预后的相关性

Expression of serum circMTO1 in patients with hepatitis B associated cirrhosis and its correlation with degree of liver fibrosis and prognosis

目的分析乙型肝炎相关性肝硬化患者血清环状RNA线粒体tRNA翻译优化因子1(circMTO1)表达水平及其与肝纤维化程度、预后的关系。方法纳入115例乙型肝炎相关性肝硬化患者(乙肝肝硬化组)及120例慢性乙型肝炎患者(慢乙肝组)作为研究对象。根据治疗后短期预后情况,将乙肝肝硬化组患者分为预后良好组(n=84)与预后不良组(n=31)。比较乙肝肝硬化组与慢乙肝组之间、预后良好组与预后不良组之间的血清circMTO1表达水平。分析乙肝肝硬化组血清circMTO1表达水平与肝纤维化分级的相关性。采用受试者工作特征曲线分析血清circMTO1表达水平对乙肝肝硬化组患者不良预后的预测价值。结果乙肝肝硬化组患者的血清circMTO1表达水平低于慢乙肝组,预后不良组患者的circMTO1表达水平低于预后良好组(P<0.05)。乙肝肝硬化组患者血清circMTO1表达水平与肝纤维化分级呈负相关(P<0.05)。血清circMTO1表达水平预测乙肝肝硬化组患者预后不良的曲线下面积为0.747,敏感度为77.40%,特异度为77.40%,最佳截断值为0.332。结论与未发生肝硬化的慢性乙型肝炎患者相比,乙型肝炎相关性肝硬化患者的血清circMTO1表达水平降低,且其与肝纤维化程度相关。circMTO1或可作为评估乙型肝炎相关性肝硬化患者预后的分子标志物。

Objective To analyze the expression of serum circular RNA mitochondrial tRNA translation optimization 1(circMTO1)in patients with hepatitis B associated cirrhosis and its relation with the degree of liver fibrosis and prognosis.Methods A total of 115 patients with hepatitis B associated cirrhosis(the hepatitis B cirrhosis group)and 120 patients with chronic hepatitis B(the chronic hepatitis B group)were enrolled as the research subjects.Patients in the hepatitis B cirrhosis group were assigned to favorable prognosis group(n=84)or adverse prognosis group(n=31)according to short-term prognosis after treatment.Serum circMTO1 expression was compared between the hepatitis B cirrhosis group and the chronic hepatitis B group,and between the favorable prognosis group and the adverse prognosis group.The correlation of serum circMTO1 expression with classification of liver fibrosis was analyzed in the hepatitis B cirrhosis group.The receiver operating characteristic curve was used to analyze the predictive value of serum circMTO1 expression on adverse prognosis in patients of the hepatitis B cirrhosis group.Results Patients in the hepatitis B cirrhosis group obtained a lower serum circMTO1 expression as compared with the chronic hepatitis B group,and serum circMTO1 expression was lower in the adverse prognosis group than in the favorable prognosis group(P<0.05).Serum circMTO1 expression negatively correlated with classification of liver fibrosis in patients of the hepatitis B cirrhosis group(P<0.05).Area under the curve of serum circMTO1 expression for predicting adverse prognosis in patients of the hepatitis B cirrhosis group was 0.747,the sensitivity was 77.40%,the specificity was 77.40%,and the optimal cut-off value was 0.332.Conclusion Compared with chronic hepatitis B patients without suffering from cirrhosis,patients with hepatitis B associated cirrhosis obtain a decreased serum circMTO1 expression,and it is related to the degree of liver fibrosis.Prognosis of patients with hepatitis B associated cirrhosis can be evaluated by circMTO1,which can be regarded as a molecular marker.