独活寄生汤对大鼠膝骨关节炎进展的影响及调控巨噬细胞极化研究

Effects of Duhuo Jisheng Decoction on the progression of knee osteoarthritis in rats and the regulation of macrophage polarization

为了阐明独活寄生汤在碘乙酸钠(MIA)诱导大鼠膝骨关节炎模型中的作用,探讨独活寄生汤对M1型巨噬细胞/M2型巨噬细胞极化的影响。论文将40只大鼠随机分为4组,分别为正常组、模型组、阳性药物组和独活寄生汤组。采用关节腔注射MIA的方法制备KOA大鼠模型,使用HE染色及Mankin评分法观察关节软骨形态学变化并进行损伤程度评分,ELISA法检测血清中TNF–α和IL–10含量。实验结果表明:与模型组相比,独活寄生汤组及阳性药物组的大鼠软骨组织细胞结构变化较为轻微;Mankin评分明显改善,并且在大鼠血清中IL–10表达显著增加(P<0.01,P<0.05),同时TNF–α表达显著降低(P<0.01)。独活寄生汤显著延缓大鼠膝骨关节炎的发展过程,其作用机制与调控M1/M2巨噬细胞极化有关。

To clarify the role of Duhuo Jisheng Decoction in sodium iodoacetate(MIA)-induced knee osteoarthritis model in rats,and to explore the effect of Duhuo Jisheng Decoction on M1 macrophage/M2 macrophage polarization,40 KOA rats were randomly divided into 4 groups:normal group,model group,positive drug group and Duhuo Jisheng Decoction group.The KOA rats were induced by intraarticular injection of MIA.The morphological changes of articular cartilage and the degree of injury were observed using HE staining and Mankin scoring method.Compared with the model group,the Duhuo Jisheng Decoction group and the positive drug group had slight changes in the cell structure of rat cartilage tissue,significantly improved Mankin scores,significantly increased expression of IL-10 in rat serum(P<0.01,P<0.05),and significantly decreased expression of TNF-α(P<0.01).Duhuo Jisheng Decoction can significantly delay the development of knee osteoarthritis in rats,and its mechanism is related to the regulation of M1/M2 macrophage polarization.