活体检测吲哚-3-乙酸的电化学传感器研究

Research on electrochemical sensor for in vivo detection of indole-3-acetic acid

吲哚-3-乙酸(IAA)作为一种重要的植物激素,在植物生长发育的整个过程中起着至关重要的作用。本文根据植物活体检测的实际需要,发展了适合植物茎秆活体检测的四阵列电极及用于植物果实活体检测的六阵列电极,通过一步法在铂(Pt)丝工作电极上沉积铂纳米颗粒(PtNPs)和还原氧化石墨烯(rGO)纳米复合膜,从而增强催化效果,提高生物传感器的灵敏度。实验结果表明,所研制的传感器在IAA浓度0.1~200μmol/L范围内,具有良好的线性关系,相关系数为R~2=0.986,检测限为0.01μmol/L(S/N=3)。进一步利用所制备的传感器活体检测了黄瓜茎秆和黄瓜果实(CF)内的IAA含量,表明该传感器在植物生理状态的活体检测中具有重要应用前景。

As an important plant hormone,indole-3-acetic acid(IAA)plays a crucial role in the whole process of plant growth and development.According to the actual needs of in vivo detection of plants,a four-array electrode suitable for plant stem and a six-array electrode for plant fruit are developed.Platinum nanoparticles(PtNPs)and reduced graphene oxide(rGO)nanocomposite film are deposited on the platinum wire working electrode by one-step method,thereby enhancing the catalytic effect and improving the sensitivity of the biosensor.The experimental results show that the developed sensor has a good linear relationship between the IAA concentration range of 0.1~200 μmol·L~(-1),correlation coefficient R~2=0.986,and limitation of detection is 0.01 μmol·L~(-1)(S/N=3).The prepared sensor is further used to detect the IAA content in cucumber stem and cucumber fruit in vivo,indicating that the sensor has important application prospects in the in vivo detection of plant physiological states.