叔丁醇暴露对HSPA1L敲除型雄性小鼠甲状腺的影响

Effects of tert-butanol exposure on thyroid in HSPA1L knockout male mice

以HSPA1L基因敲除(HSPA1L^(-/-))雄性小鼠为研究对象,观察甲状腺组织形态和细胞凋亡情况,分析Caspase-3蛋白表达,探讨叔丁醇(TBA)的毒性以及HSPA1L在此过程中的作用机制.结果表明,HSPA1L^(-/-)小鼠甲状腺的重量随TBA剂量增加而显著降低(P<0.05);甲状腺上皮滤泡细胞随TBA剂量增加而增大,高剂量组甲状腺滤泡甚至发生融合现象;甲状腺细胞凋亡增强,甲状腺组织Caspase-3表达量极显著增加(P<0.01).HSPA1L^(-/-)小鼠对TBA暴露更为敏感,TBA造成的伤害显著增强,提示HSPA1L可能通过负调控Caspase-3表达,抑制细胞凋亡,维持细胞稳态.

In this study,male mice with HSPA1L gene deletion were studied tOobserve the thyroid tissue morphology and cell apoptosis,analyze the expression of Caspase-3 protein,and explore the toxicity of TBA and the potential mechanism of HSPA1L.The results showed that the thyroid weight of HSPA1L^(-/-)mice decrease significantly with the increase of TBA dose(P<0.05).Thyroid epithelial follicular cells enlarged with the increase of TBA dose,and follicular fusion even occured in the high dose group.Cell apoptos of thyroid tissue were significantly enhanced.The expression of Caspase-3 in thyroid tissue was very significantly increased(P<0.01).This study showed that HSPA1L^(-/-)mice were more sensitive to TBA exposure,and the damage caused by TBA was significantly enhanced,suggesting that HSPA1L had strong protective effects on inhibiting apoptosis and maintaining cell homeostasis.