番茄颈腐根腐病病原菌鉴定及抗性品种筛选

Identification of Pathogen Causing Fusarium Crown and Root Rot and Screening Resistant Varieties in Tomato

番茄颈腐根腐病是设施番茄冬春生产中最严重的病害之一,严重威胁我国设施番茄的安全生产。近年来番茄颈腐根腐病在我国番茄主产区发生日益严重,培育抗病品种是防治该病最经济有效的方法。本试验采集典型症状的发病植株进行病原菌分离纯化,综合运用形态学观察、分子生物学鉴定和致病性测定方法,确定病原菌为尖孢镰刀菌番茄颈腐根腐病专化型(Fusarium oxysporum f.sp.radicis-lycopersici,Forl)。之后利用分离的病原菌通过苗期人工接种鉴定结合抗性基因分子标记方法,对32个番茄商品种进行抗颈腐根腐病鉴定分析,结果显示有18个表现抗病,与分子标记Frl-ZL的鉴定结果一致,可用于番茄抗颈腐根腐病育种或生产。

Fusarium crown and root rot(FCRR)is one of the most serious diseases threating protected tomato production in China.In recent years,the incidence of FCRR is increasing in the main producing areas of tomato in China.Cultivating resistant varieties is the most cost-effective method for preventing the disease.In this study,the pathogen was isolated from diseased tomato plants by tissue-isolation method.Based on morphological characteristics,molecular identification and pathogenicity test,the causal pathogen was identified as Fusarium oxysporum f.sp.radicis-lycopersici(Forl).To explore the resistant varieties against Forl in production,the resistance of 32 varieties to Forl were identified through artificial inoculation at seedling stage and molecular marker-assisted identification of Frl gene.The results of inoculation showed 18 materials with resistance to FCRR,which was consistent with the identification results using molecular marker Frl-ZL.These selected varieties could be used in breeding or production of tomato with FCRR resistance.