分子印迹电化学方法优化花生壳中木犀草素提取工艺

Optimization of Extraction Process of Luteolin from Peanut Shells Based on Molecular Imprinted Electrochemical method

采用循环伏安(cyclic voltammetry,CV)原位聚合法,以对氨基苯酚(p-aminophenol,p-AP)和3,4-乙烯二氧噻吩(3,4-ethylenedioxythiophene,EDOT)为双功能单体,制备分子印迹聚合物(molecularly imprinted polymer,MIP),用于木犀草素电化学传感器的构建,并进行表征。采用酶解辅助超声波法提取花生壳中木犀草素,结合Box-Behnken响应面法优化提取工艺。结果表明,p-AP和EDOT实现了CV共聚,MIP对木犀草素展示出良好的电催化识别能力;优选最佳提取工艺为酶解时间81 min,酶解温度45℃,超声时间60 min,超声温度45℃,提取率为0.8620%(RSD=2.287%),与HPLC方法测定结果接近(0.8922%),说明所建立检测方法准确可靠。

Molecularly imprinted polymer(MIP)was prepared by cyclic voltammetry(CV)in-situ polymerization with p-aminophenol(p-AP)and 3,4-ethylenedioxythiophene(EDOT)as bifunctional monomers and Luteolin as template molecules for the construction of electrochemical sensors.Enzymatic hydrolysis-assisted ultrasonic method was used to extract luteolin from peanut shells,and the extraction process was optimized by Box-Behnken response surface method.The results showed that p-AP and EDOT achieved CV copolymerization on the electrode surface,and MIP showed good electrocatalysis and recognition ability for luteolin.The optimal extraction process was as follows:enzymatic hydrolysis time was 81 min,enzymatic hydrolysis temperature was 45℃,ultrasonic time was 60 min,ultrasonic temperature was 45℃,and the extraction yield of Luteolin reached 0.8620%(RSD%=2.525%),which was close to that obtained by HPLC method(0.8922%),indicating that the established detection method was accurate and reliable.