miR-129-2对三阴性乳腺癌细胞迁移和侵袭能力的调节作用

Regulation of miR-129-2 on migration and invasion ability of triple negative breast cancer cells

目的:检测miR-129-2在三阴性乳腺癌(TNBC)患者癌组织中的表达水平,分析miR-129-2的表达与患者临床资料的关系,并探究其对癌细胞迁移和侵袭能力的影响。方法:荧光实时定量PCR(RT-qPCR)法检测TNBC癌组织、癌旁组织以及细胞株中miR-129-2的表达水平,统计学方法分析miR-129-2的表达水平与患者临床资料的相关性,采用划痕实验及Transwell实验研究miR-129-2对癌细胞迁移和侵袭能力的影响。结果:miR-129-2在40例TNBC患者癌组织及MDA-MB-231细胞中表达下调(P<0.05,P<0.01),且miR-129-2的表达水平与年龄、是否绝经无关,与肿瘤大小、分期、淋巴结转移有关;划痕实验结果显示:过表达miR-129-2使细胞迁移距离减少,划痕愈合率均下降,迁移能力减弱(P<0.01);Transwell侵袭实验显示:过表达miR-129-2能减少穿过小室膜的细胞数量,明显抑制癌细胞的侵袭能力(P<0.01)。下调miR-129-2的水平使MDA-MB-231细胞迁移能力和侵袭能力增强。结论:miR-129-2在TNBC中低表达,miR-129-2的表达与病情严重程度相关,miR-129-2的表达水平影响TNBC癌细胞的迁移和侵袭能力。

Objective:To detect the expression level of miR-129-2 in cancer tissues of patients with triple-negative breast cancer(TNBC),analyze the relationship between the expression of miR-129-2 and clinical data of patients,and explore its influence on the migration and invasion of cancer cells.Methods:Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the expression level of miR-129-2 in TNBC cancer tissues,adjacent tissues and cell lines,and the correlation between the expression level of miR-129-2 and clinical data of patients was analyzed statistically.The effects of miR-129-2 on the migration and invasion of cancer cells were investigated by scratch assay and Transwell assay.Results:miR-129-2 expression was down-regulated in cancer tissues of 40 TNBC patients and MDA-MB-231 cells(P<0.05,P<0.01),and the expression level of miR-129-2 was not related to age and menopause,but was related to tumor size,stage and lymph node metastasis.The results of scratch test showed that overexpression of miR-129-2 reduced the migration distance of cells,the healing rate of scratches decreased,and the migration ability weakened(P<0.01).Transwell invasion assay showed that overexpression of miR-129-2 could reduce the number of invasion cells and significantly inhibit the invasion ability of cancer cells(P<0.01).Down-regulating the level of miR-129-2 enhanced the migration and invasion ability of MDA-MB-231 cells.Conclusion:The expression of miR-129-2 is low in TNBC,and the expression of miR-129-2 is related to the severity of the disease.The expression level of miR-129-2 affects the migration and invasion ability of TNBC cancer cells.