低聚果糖对AOM/DSS诱导的结肠炎相关结直肠癌小鼠辅助性T细胞的调控作用

Regulatory effect of fructooligosaccharide on helper T cells in AOM/DSS induced colitis-associated colorectal cancer mice

目的探讨益生元低聚果糖(FOS)对结肠炎相关结直肠癌(CAC)小鼠肠道辅助性T细胞的调节作用。方法将6周龄SPF级雄性C57BL6小鼠(体重18~20 g)随机分为对照组、模型组和干预组,每组10只。通过偶氮甲烷、葡聚糖硫酸钠构建CAC小鼠模型,干预组每日使用2 mg/kg b.w.FOS灌胃,持续10周。造模期间监测各组小鼠体重变化,造模结束后测量结肠长度和肿瘤数目。采用微球免疫分析法检测血清Th1/Th2/Th17相关细胞因子水平,流式细胞术分析肠系膜淋巴结内Th1、Th2、Th17细胞亚群比例。结果与模型组相比,干预组小鼠结肠长度显著增加(t=3.106,P=0.0064),肿瘤数目显著减少(U=15.000,P=0.0111),血清中IL-17A(t=3.504,P=0.0088)、TNF-α(t=2.381,P=0.0300)等促炎细胞因子水平降低,肠系膜淋巴结Th17细胞比例显著降低(t=6.031,P=0.0027),Th1/Th2显著升高(t=2.419,P=0.0387)。结论FOS可调控CAC模型小鼠结肠内辅助性T细胞的分化,抑制肿瘤的发生。

Objective To observe the effect of prebiotic fructooligosaccharide(FOS)on intestinal helper T cells in colitisassociated colorectal cancer(CAC)mice.Methods Six-week-old SPF male C57BL6 mice were randomly divided into the control group(Control group),model group(AOM/DSS group),and intervention group(AOM/DSS-FOS group).The CAC model was established by using azoxymethane and dextran sulfate sodium.The intervention group was given 2 mg/kg b.w.FOS by gavage for 10 weeks.The weight change in each group was monitored during the modeling period,and the colon length and tumor number were measured after the modeling.Serum Th1/Th2/Th17-related cytokines were detected with Cytometric Beads Array,and the proportions of Th1,Th2 and Th17 cells in mesenteric lymph nodes were analysed by flow cytometry.Results Compared with the AOM/DSS group,the colon length(t=3.106,P=0.0064)increased,the number of tumors(U=15.000,P=0.0111)decreased,the serum levels of proinflammatory cytokines such as IL-17A(t=3.504,P=0.0088)and TNF-α(t=2.381,P=0.0300)significantly decreased,the proportion of Th17 cell(t=6.031,P=0.0027)in mesenteric lymph nodes significantly decreased,and Th1/Th2(t=2.419,P=0.0387)significantly increased in the intervention group.Conclusion Fructooligosaccharide can regulate the differentiation of helper T cells in the colon of CAC model mice,and inhibit the occurrence of tumors.