高效液相色谱法同时检测化妆品中视黄醇及其衍生物

Simultaneous determination of retinol and its derivatives by high performance liquid chromatography

建立了检测化妆品中视黄醇及其衍生物等16种组分含量的高效液相色谱分析方法。分离检测16种同类物质及同分异构体的优化条件为:采用Kromasil 100-5-C_(18)色谱柱(250 mm×4.6 mm, 5μm)分离,以甲醇为流动相A,四氢呋喃为流动相B, 10 mmol/L磷酸二氢钠溶液为流动相C,梯度洗脱,流速为1.0 mL/min,柱温为25℃。使用二极管阵列检测器,标准曲线法定量。16种组分的线性相关系数均大于0.999,在3个添加水平下,16种组分的平均回收率为91.2%~107.8%,相对标准偏差为0.5%~4.0%。该方法可以为化妆品质量监督提供有效的技术支持。

A high performance liquid chromatography(HPLC)method for the determination of 16 components including retinol and its derivatives in cosmetics was established.The optimized conditions for the separation and detection of the 16 components were as follows:separated by a Kromasil 100-5-Cis column(250 mmx4.6 mm,5μm),and gradient elution with methanol as mobile phase A,tetrahydrofuran as mobile phase B and 10 mmol/L sodium dihydrogen phosphate solution as mobile phase C,with a flow rate of 1.0 mL/min and the column temperature of 25°C.The analysis was completed using photo-diode array detector and quantified by standard curve method.The results showed that the linear correlation coefficients of the 16 components were greater than 0.999,the average recoveries were 91.2%-107.8%,and the relative standard deviations were 0.5%-4.0%at three spiked levels.This method can provide effective technical support for cosmetics quality supervision.