3种铁核桃对云斑白条天牛的抗性研究

Resistance of Three Varieties Juglans sigillata to Batocera lineolata

为筛选出对云斑白条天牛Batocera lineolata具有抗性的铁核桃Juglans sigillata品种,找出影响铁核桃抗虫性的主要化学物质,在细香核桃J.sigillata cv.‘Xixiang’、龙佳核桃J.sigillata cv.‘Niangqing’×J.sigillata cv.‘Yangbidapao’和娘青核桃J.sigillata cv.‘Niangqing’3种铁核桃苗上接种云斑白条天牛成虫,比较受害面积并测定为害前后3个铁核桃品种树皮的营养物质、次生代谢物质含量、保护酶和蛋白酶抑制剂活性差异,选出抗虫性最强的铁核桃品种。结果表明,娘青核桃受到云斑白条天牛成虫的为害面积最小,为0.0007 m^(2),影响铁核桃树皮抗虫性的主要营养和次生代谢物质是可溶性蛋白质和总酚;影响铁核桃树皮抗虫性的主要保护酶和蛋白酶抑制剂分别是超氧化物歧化酶和胰凝乳蛋白酶抑制剂,娘青核桃的抗虫性综合得分最高。由此得出,3个铁核桃品种中娘青核桃的抗虫性最强,可溶性蛋白质、总酚、超氧化物歧化酶和胰凝乳蛋白酶抑制剂是影响铁核桃树皮抗虫性的主要物质。

To screen out walnut varieties with excellent resistance to the Batocera lineolata.Find out the main chemicals that affect walnut insect resistance.Inoculate adults B.lineolata on the walnut Xixiang;Longjia and Niangqing seedlings.Compare the feeding area of different walnut varieties and measure the differences in nutrients,secondary metabolites,protective enzymes and protease inhibitors activities of bark of the three walnut varieties before and after affected.The result shows that the bark of Niangqing walnuts was the least affected by adult B.lineolata,which was 0.0007 m^(2),the main nutrients and secondary metabolites affecting insect resistance of walnut bark were soluble protein and total phenols.The main protective enzymes and protease inhibitors affecting the insect resistance of walnut are superoxide dismutase and chymostatin in-hibitor,among which the comprehensive score of insect resistance of the bark of Niangqing walnut is the highest.In conclusion,Niangqing walnut had the strongest insect resistance.Soluble proteins,total phenols,superoxide dismutase and chymotrypsin inhibitors are the main substances affecting the insect resistance of walnut bark.The result laid the foundation for the breeding of new walnut varieties and the study of plant resistance mechanism.