任豆组培再生研究

Primary Study on Tissue Culture Regeneration Technology of Zenia insignis

为完善任豆Zenia insignis组培快繁技术,研究采用微枝扦插的组培再生方式,探讨了基本培养基中NO-3、K+、Ca2+不同含量,以及不同植物生长调节剂种类与浓度对任豆组培芽增殖与生根效果的影响,结果表明:(1)任豆种子用75%酒精、0.1%升汞分别浸泡灭菌10,30 min,再用始温为85℃无菌水浸种24 h效果最好,种子萌芽快,种子发芽率可达92.16%;(2)RD基本培养基中NO^(-)_(3)、K^(+)、Ca^(2+)含量分别以600,360,330mg/L为最优配比,最佳继代培养基配比为RD+6-BA 0.2 mg/L+NAA 0.01 mg/L,20 d增殖系数可达3.32;(3)NAA对任豆组培芽生根以及生长有极显著的影响,最佳生根培养基配比为1/2 MS+NAA 0.5 mg/L+AC 1.0 g/L,生根率可达100%,且根系粗壮发达,组培苗生长健壮,叶片浓绿舒展。

In order to improve the tissue culture regeneration technology of Zenia insignis,the effects of different contents of NO-3,K+,and Ca2+in basic medium and different types and concentrations of plant growth regulators on the proliferation and rooting of Z.insignis were investigated by means of micro-cuttings.The results showed as follows:(1)The seeds of Z.insignis were soaked with 75%alcohol for 10 min and 0.1%HgCl2 for 30 min,respectively,and then soaked with sterile water at 85℃for 24 h,which had the best effect,rapid seed germination and seed germination rate can reach 92.16%.(2)The optimal ratio of NO^(-)_(3),K^(+)and Ca^(2+)in RD basic medium was 600 mg/L,360 mg/L and 330mg/L,respectively.The optimal subculture medium was RD+6-BA 0.2 mg/L+NAA 0.01 mg/L,and the proliferation coefficient reached 3.32 at 20 days.(3)NAA had a very significant effect on the rooting and growth of Z.insignis Chun.The optimal rooting medium was 1/2MS+0.5 mg/L NAA+AC 1.0 g/L,the rooting rate could reach 100%,and the roots were robust and developed,the tissue culture seedlings grew robustly,and the leaves were dark green and stretched.