基于响应面法优化芡实多糖提取工艺及其体外抗氧化研究

Extraction Technology of Polysaccharide from Euryale ferox Salisb by Response Surface Methodology and Antioxidant Activity in Vitro

采用单因素实验和响应面法对芡实多糖的浸提工艺进行优化,并探讨其体外抗氧化活性。以芡实多糖提取率为指标,分别考察浸提温度、浸提时间、料液比、pH和乙醇浓度5个单因素对芡实多糖提取率的影响。通过响应面法对4个主要工艺参数进行优化,得到芡实多糖最佳提取工艺条件为浸提温度95℃,浸提时间5.3 h,料液比1∶24 g/mL,乙醇浓度93%。在最佳提取工艺条件下,芡实多糖提取率为0.65%。体外抗氧化活性结果表明,在0.1~10.0mg/mL范围内,芡实多糖具有较好的体外抗氧化性能,芡实多糖对DPPH自由基、超氧阴离子和羟基自由基的最大清除能力分别为82.10%、58.91%和71.07%,芡实多糖也具有较好的总还原力。该研究结果可为创制以芡实多糖为主要原料的功能食品和营养补充剂提供理论基础。

Single factor testing and response surface methodology were employed to optimize the extraction process of polysaccharide from Euryale ferox Salisb,and the in vitro antioxidant activities were further investigated.Taking the extraction rate of polysaccharide from Euryale ferox Salisb as the index,the effects of five single factors including extraction temperature,extraction time,solid-liquid ratio,pH and ethanol concentration on the extraction rate of Euryale ferox Salisb were investigated.Consequently,response surface method was performed to optimize these four main process parameters.The optimal extraction conditions of Euryale ferox Salisb polysaccharide were obtained as follows:the extraction temperature is 95℃,the extraction time 5.3 h,the solid-liquid ratio 1∶24,and the ethanol concentration 93%.The extraction rate of polysaccharide from Euryale ferox Salisb had the highest rate of 0.65%under the optimal extraction conditions.The results of in vitro antioxidant activity showed that in the range of 0.10~10.0 mg/mL,Euryale ferox Salisb polysaccharide had good antioxidant performance on the scavenging capacities of scavenging DPPH radical,superoxide anion free radical and hydroxyl radical with the results of 82.10%,58.91%and 71.07%respectively.Euryale ferox Salisb polysaccharide also had good total reducing power.The results of this study provided a theoretical basis for the creation of functional food and nutritional supplements based on Euryale ferox Salisb polysaccharide.