6种铁线莲种质SSR遗传多样性分析

Analysis on Genetic Diversity of Six Clematis Germplasma by SSR Markers

通过对河南25个居群的6种铁线莲种质进行遗传多样性分析,研究铁线莲种质资源分布和种群间的亲缘关系.筛选出6对引物,利用SSR标记技术对120个铁线莲样品进行遗传多样性分析.结果表明,25个居群的120个铁线莲的PIC值范围为0.619~0.738,有效等位基因数(Ne)为1.577±0.233,遗传相似系数在0.58到1.03之间,6对引物扩增出33条带,平均每个引物5.5条.同一种质的铁线莲优先聚为一类,同一种质亲缘关系更近,地理位置较近的亲缘关系也较近,25个居群的铁线莲具有丰富的遗传多样性.研究结果可为后续铁线莲种质创新和新品种选育提供参考.

This research aims to conduct a study on the resource distribution of clematis germplasms and the genetic relationship among populations by analysis on the genetic diversity of six clematis germplasms chosen from twenty-five populations in Henan Province.The researchers selected six pairs of primers and analyzed the genetic diversity of 120 Clematis samples by SSR markers.The findings show that the PIC values of 120 clematis from twenty-five populations range from 0.619 to 0.738,the number of effective alleles(Ne)is 1.577±0.233,and the genetic similarity coefficients range from 0.58 to 1.03,and the six pairs of primers amplify thirty-three bands with an average of 5.5 bands per primer.The results show that clematis of the same germplasm preferentially cluster into one group,clematis of the same germplasm presents closer genetic relationship,and the germplasms with closer geographical location also presents closer genetic relationship,and the twenty-five populations of clematis present rich genetic diversity.These results provide reference for the subsequent germplasm innovation and new variety breeding of clematis.