黄河三角洲盐碱地耐盐促生菌株的筛选与功能评价

Screening and Functional Evaluation of Salt Tolerant and Growth Promoting Strains in Saline Alkali Land of the Yellow River Delta

通过对黄河三角洲盐碱地植物根际土壤的筛选,获得47株耐盐菌株,其中9株具有促生效果。结合产酸、耐碱、耐不同盐碱土的能力,最终获得1株耐盐菌株GJY-3。GJY-3在氯化物盐土、硫酸盐土、重碳酸盐土中的耐受程度分别为5%、5%、1%,酸碱耐受范围为3.15~10.5,无机磷溶解量为16.71 mg/L,吲哚乙酸的合成能力为31.33 mg/L,同时具备固氮、解钾功能。通过生理生化分析和16s rDNA鉴定,确定该菌株为地衣芽孢杆菌。通过盆栽试验发现:GJY-3在氯化钠盐碱土的促生效果优于硫酸钠盐碱土的;小白菜产量以及土壤脲酶、蔗糖酶、过氧化氢酶活性分别增加17.54%、91.59%、32.24%、5.11%。试验结果表明,GJY-3可用作盐碱地土壤改良菌株。

By screening the rhizosphere soil of plants in the saline alkali land of the Yellow River Delta,47 salt tolerant strains are obtained and of which 9 strains have a promoting effect on growth.By combining the abilities of acid production,alkali resistance,and tolerance to different saline alkali soils,one salt tolerant strain GJY-3 is finally obtained.The tolerance of GJY-3 in chloride soil,sulfate soil and bicarbonate soil is 5%,5%,and 1%,respectively.The acid-base tolerance range is 3.15-10.5,the inorganic phosphorus dissolution amount is 16.71 mg/L,and the synthesis ability of indoleacetic acid is 31.33 mg/L.The strain also has nitrogen fixation and potassium solubilization functions.Through physiological and biochemical analysis and 16s rDNA identification,the strain is identified as Bacillus licheniformis.The pot experiment show that GJY-3 has a better promoting effect on growth in sodium chloride saline alkali soil than in sodium sulfate saline alkali soil.The yield of pakchoi and the activities of soil urease,sucrase,and catalase increased by 17.54%,91.59%,32.24%,and 5.11%,respectively.The experimental results indicate that GJY-3 can be used as a strain for improving saline alkali soil.