高效液相色谱法定量分析食用油中的黄曲霉毒素B_(1)

Quantitative Determination of Aflatoxins B_(1) in Edible Oil by High Performance Liquid Chromatography

目的:建立高效液相色谱测定食用油中黄曲霉毒素B_(1)的方法。方法:样品用甲醇水提取,经黄曲霉毒素B_(1)免疫亲和柱净化,用甲醇洗脱,洗脱液用柱后碘衍生法上机测定。结果:该方法线性回归方程为Y=27 217.3X,相关系数R~2=0.999 6,回收率在96.8%~101.2%,方法重复性的RSD为0.6%,质控样的Z值为0.5,检出限为0.029μg·kg^(-1),定量限为0.098μg·kg^(-1)。结论:该方法线性关系良好,回收率优良,质控样、检出限、定量限均符合要求,具有简单、快速、高效、节约试剂耗材的特点。

Objective:In order to establish the determination method of aflatoxins B_(1) in edible oil by high performance liquid chromatography.Method:The samples were extracted with methanol water,purified by aflatoxins B_(1) immunoaffinity column,eluted with methanol,and determined by post-column iodine derivatization.Result:The linear regression equation was Y=27217.3X,the correlation coefficient was R2=0.9996.The recovery rate of method was from 96.8%to 101.2%,the RSD of reproducibility was 0.6%,the Z value of quality control samples was 0.5,and the detection limit was 0.029μg·kg^(-1),the limit of quantification was 0.098μg·kg^(-1).Conclusion:The method has good linearity,good recovery,quality control sample,detection limit,quantitative limit are in line with the requirements,with simple,fast,efficient and save reagent consumables characteristics.