基于网络药理学探讨木蝴蝶治疗咽炎疾病的作用机制

Molecular Mechanism of Oroxyli Semen in Treating Pharyngitis Based on Network Pharmacology

目的:分析中药木蝴蝶有效活性成分及其治疗咽炎疾病的分子机制。方法:通过广泛应用的中药系统药理学数据库及分析平台(TCMSP)数据库系统、Genecards数据库系统、在线《人类孟德尔遗传》(OMIM)数据库系统查找木蝴蝶有效活性成分及其作用靶点蛋白和人类咽炎疾病相关的作用靶点,并通过Cytoscape工具构建木蝴蝶有效活性成分-咽炎疾病靶点的网络图;通过String系统构建蛋白质-蛋白质相互作用(PPI)互作网络关系图,应用Metascape线上系统进行相应基因本体(GO)功能注释、京都基因与基因组百科全书(KEGG)通路富集分析。结果:经过筛选,木蝴蝶中的槲皮素、汉黄芩素、β-谷固醇、黄芩素、豆甾醇、芦荟大黄素、鞣花酸等关键活性成分,有可能通过作用在细胞肿瘤抗原p53(TP53)、丝氨酸/苏氨酸蛋白激酶1(AKT1)、肿瘤坏死因子(TNF)、白细胞介素-6(IL-6)、前列腺素内过氧化物合成酶2(PTGS2)、胱天蛋白酶3(CASP3)、白细胞介素-1β(IL-1β)、血管内皮生长因子A(VEGFA)、原癌基因(MYC)、基质金属蛋白酶9(MMP9)等核心基因表达的蛋白上参与咽炎疾病的治疗。KEGG富集分析所涉及的通路包括癌症通路、巨细胞病毒感染、PI3K-Akt信号通路(PI3K-Akt signaling pathway)等。结论:木蝴蝶可通过多活性成分、多作用靶位、多通路的复杂机制发挥调节机体免疫抗炎作用,发挥对咽炎疾病的治疗作用,同时可能通过抗肿瘤机制防止咽炎疾病向更严重的咽部疾病转化。本研究为木蝴蝶治疗咽炎疾病提供了理论依据。

Objective To search molecular mechanism of Oroxyli Semen in Treating pharyngitis.Methods The traditional chinese medicine systems pharmacology database and analysis platform(TCMSP)and Genecards database Online Mendelian Inheritance of Man(OMIM)database system were used to screen out potential chemically active substances and the targets related to human pharyngitis separately;Then,the network of active substances and disease targets was pictured through Cytoscape.protein-protein interaction(PPI)core genes was calculated by String database platform;Perform gene ontology(GO)enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis was analysis by Metascape database.Results After screening,many key chemical components in Oroxyli Semen might be effective for pharyngitis treatment through cellular tumor antigen p53(TP53),AKT serine/threonine kinase 1(AKT1),tumor necrosis factor(TNF),interleukin-6(IL-6),prostaglandinendoperoxide synthase 2(PTGS2),caspase 3(CASP3),interleukin 1 beta(IL-1β),vascular endothelial growth factor A(VEGFA),myelocytomatosis oncogene(MYC),matrix metallopeptidase 9(MMP9)namely quercetin,wogonin,beta-sitosterol,baicalein,Stigmasterol,aloe-emodin,ellagic acid etc.KEGG enrichment analysis results include Pathways in cancer,Human cytomegalovirus infection,PI3K-Akt signaling pathway,et al.Conclusion Oroxyli Semen can treat pharyngitis through the complex action mechanism of multiple active ingredients,multiple targets and multiple pathways,and at the same time,it may prevent pharyngitis diseases from transforming into more serious pharyngeal diseases through anti-tumour mechanisms.This study provides a theoretical basis for the treatment of pharyngitis disease by Oroxyli Semen.