广金钱草黄酮成分在肾结石大鼠中的代谢研究

Metabolism of Total Flavonoids of Desmodium Styracifolium in Rats with Calcium Oxalate Renal Stones

目的利用超高效液相色谱串联电喷雾飞行时间质谱(UPLC-Q-TOF-MS/MS)分析广金钱草黄酮成分在肾结石大鼠中的代谢途径和代谢产物。方法建立SD大鼠肾结石造模,灌胃给予肾结石大鼠广金钱草总黄酮,采用UPLC-Q-TOF-MS/MS分析黄酮成分在肾结石大鼠模型的血浆、尿液和粪便中的代谢变化,通过TOF/MS得到的准确相对分子质量,多级质谱裂解信息,结合对照品及相关文献报道对黄酮成分的裂解规律总结结果对比鉴定,对血浆、尿液和粪便中总黄酮成分和代谢产物进行鉴定。结果广金钱草总黄酮给药后,在大鼠血浆中鉴定出5种原型成分和3种夏佛塔苷的同分异构体,16种以血浆中原型成分代谢得到的产物;从尿液中鉴定出12种原型成分和21种以尿液中原型成分代谢得到的产物;从粪便中鉴定10种原型成分和43种以粪便中原型成分代谢得到的产物。结论阐述了广金钱草总黄酮在大鼠体内的代谢变化情况,为该药材今后的药效物质基础研究提供了理论参考。

OBJECTIVE To explore the metabolic pathways and metabolites of total flavonoids of Desmodium styracifolium(TFDS)in rats with calcium oxalate renal stones by ultrahigh performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS).METHODS Calcium oxalate renal stones model was established in SD rats,and the rats were treated by TFDS by gavage.UPLC-Q-TOF-MS/MS was used to analyze the metabolic changes of flavone components in plasma,urine and feces of rats with calcium oxalate renal stones.The total flavone components and metabolites in plasma,urine and feces were identified by accurate relative molecular weight and multistage mass spectrometry cleavage fragments information obtained from TOF/MS and combined with the reference substance and the related literature reports.RESULTS After treated by TFDS,5 compounds were prototype constituents,3 compounds were isomers of schaftoside,and 16 components obtained by metabolism of prototype components,which were found in the plasma of rats.12 prototype components of TFDS and 21 metabolized components were identified from urine.There were 10 prototype components in feces,and 43 products were obtained by metabolism of prototype components in feces.CONCLUSION The metabolic changes of total flavonoids of Desmodium styracifolium in rats are reseached,which provides a theoretical reference for the basic research of Desmodium styracifolium in the future.