基于HPLC-LTQ-Orbitrap-MS/MS技术快速分析肺形草中的化学成分

Rapid Analysis of Chemical Components in Tripterospermum chinense ( Migo) H. Smith Based on HPLCLTQ-Orbitrap-MS/MS Technology

目的 建立高效、稳定的高效液相色谱-线性离子阱串联质谱联用技术(HPLC-LTQ-Orbitrap-MS/MS)方法,对肺形草全草的化学成分快速识别和鉴定。方法 采用HPLC-LTQ-Orbitrap-MS/MS对肺形草中的化学成分进行快速分析。通过HPLC-LTQ-Orbitrap-MS/MS扫描得到的化合物相对分子质量,对照品和多级碎片离子信息,并结合相关文献以及裂解规律,从而实现对肺形草的准确定性分析。结果 共鉴定出92种化学成分,包括黄酮类、萜类、有机酸类、蒽醌类、糖类、酯类等,其中化合物3,4-di-O-caffeoylquinic acid、(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid、(1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a, 5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid和Glc-Glc-octadecatrienoyl-sn-glycerol经全球天然产物社会分子网络系统(GNPS)数据库比对,为首次在该植物中鉴别得到。结论 建立了对肺形草中化学成分的快速定性分析的方法,为肺形草化学成分的提取分离及其药效物质基础提供科学依据,并为其质量评价和应用开发提供了一定的参考。

OBJECTIVE An efficient and stable HPLC-LTQ-Orbitrap-MS/MS method was developed to analyze and identify the chemical constituents from Tripterospermum chinense(Migo)H.Smith rapidly.METHODS The chemical components in T.chinense were analyzed by high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry(HPLC-LTQ-Orbitrap-MS/MS).HPLC-LTQ-Orbitrap-MS/MS was used to analyze the chemical components in T.chinense quickly.The accurate molecular weight,reference substance and multistage fragment ion information of the compounds obtained by HPLC-LTQ-Orbitrap-MS/MS scanning,combined with relevant literature and fragmentation regularity,were used to achieve the accurate qualitative analysis of T.chinense.RESULTS A total of 92 chemical constituents were identified,including flavonoids,terpenoids,organic acids,anthraquinones,saccharide,ester and the others.Among them,compounds 3,4-di-O-caffeoylquinic acid、(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid、(1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid and Glc-Glc-octadecatrienoyl-sn-glycerol were identified for the first time in this plant by GNPS database alignment.CONCLUSION A rapid qualitative analysis method of chemical components in T.chinense is established,which provides a scientific basis for the extraction and isolation of the chemical constituents of T.chinense and its pharmacodynamic material basis,and provides a certain reference significance for its quality evaluation and application development.