复合寡糖对设施黄瓜盛花期根际土壤代谢产物的影响

Effect of Oligosaccharide Application on Metabolites in Bulk Soil of Cucumber during the Growing Stage

寡糖具有调节作物生长发育、延缓衰老和提质增产的作用。为明确寡糖对设施黄瓜根际土壤代谢物的影响,揭示其作用机理,本研究采用大棚试验,于设施黄瓜盛花期、盛果期和拉秧期分别采集根际土壤样本,通过气相色谱-质谱法(GC-MS)比较分析清水对照(CK)与复合寡糖处理(KP)组代谢产物的差异。结果表明,与CK组相比,KP组在黄瓜盛花期根际土壤代谢物差异较大,酸性物质含量增加。其中,壬酸为上调最多的物质,差异倍数约为2.795,而杂环化合物和糖类物质含量下降,且杂环化合物含量下降最多,差异倍数约为0.002。与京都基因和基因组百科全书(KEGG)数据库对比分析,KP与CK处理在代谢途径的差异占比达到83.33%。结合差异代谢物富集分析认为,设施黄瓜盛花期施用寡糖,可通过上调硫辛酸通路增加其根际土壤中壬酸含量,而杂环化合物和糖类物质含量的下降可能与细菌趋化性代谢、果糖和甘露糖代谢这两个通路的表达下调有关。

The application of oligosaccharides has been shown to have effects in regulating crop growth,delaying senescence and improving resistance.In order to investigate the mechanism and reveal the effects of oligosaccharides on metabolites in the rhizosphere soil surrounding the plant,a cucumber greenhouse experiment was conducted in this study.The mixed-oligosaccharide(KP)was selected as the preparation treatments,while tap water control(CK)were compared as controls.During the full flowering,full fruiting and seedling stages,the rhizosphere soil samples around cucumbers were collected and analyzed to identify the differences in metabolites among different treatment groups using gas chromatography-mass spectrometry(GC-MS)method.The results showed that the cucumber treated with oligosaccharides(KP)during the full flowering period showed significant differences in bulk soil metabolites when compared with the water control group(CK).Meanwhile,nonanoic acid was the most up regulated substance,with a difference multiple of 2.795.However,the amount of heterocyclic compounds and sugars decreased.The heterocyclic compounds showed the most significant reduction in substance content,by a difference multiple of 0.002.Based on the KEGG metabolic pathway analysis,there was a significant difference in metabolic pathways between KP and CK treatment,with a difference ratio of 83.33%.Based on differential metabolite enrichment analysis,it was believed that the mixed-oligosaccharides could increase the content of nonanoic acid in the rhizosphere soil by up regulating the expression of the lipoic acid pathway,while the decrease in heterocyclic compounds and carbohydrate content might be related to the down regulation of bacterial chemotaxis metabolism,fructose and mannose metabolism pathways during the full flowering stage.