湖北省肾综合征出血热宿主带毒监测及病毒分子进化特征研究

Surveillance of host virulence and molecular evolution of hemorrhagic fever with renal syndrome in Hubei Province

目的了解湖北省肾综合征出血热(HFRS)宿主动物汉坦病毒感染状况,并对其病毒分子进行进化特征分析,为湖北省HFRS的防控提供科学依据。方法鼠肺标本用病毒DNA/RNA提取试剂盒提取组织RNA进行巢式PCR扩增,琼脂糖电泳分析及基因测序,采用DNAstar seqman软件对测序所得序列与既往毒株基因序列进行比较,使用MegAlign软件进行同源性分析,采用MEGA11.0软件Neighbor-Joining Method构建系统进化树。结果本次调查共捕获宿主动物1603只,优势宿主主要为黑线姬鼠663只,占比41.36%(663/1603),其次为黄胸鼠与褐家鼠共359只,占比22.40%(359/1603),为小家鼠10.42%(167/1603)。共检出汉坦病毒阳性鼠104只,鼠总体带毒率为6.49%,阳性鼠分类主要以黑线姬鼠和褐家鼠为主,分别占比44.23%(46/104)和20.19%(21/104)。病毒分子进化分析表明湖北省2022年测定的42份HTNV型病毒样本与湖北省HV004病毒株高度同源,12份SEOV型病毒株与WuhanRf02同属于一个分支和亚分支,均未显示明显的变异。结论湖北省啮齿动物带毒情况结果显示黑线姬鼠和褐家鼠带毒率较高,该地区存在感染风险。病毒分子进化稳定,尚未出现明显变异,同时应进一步加强病毒监测。

Objective To understand the infection status of hantavirus in host animals of hemorrhagic fever with renal syndrome(HFRS)in Hubei province,and to analyze the evolutionary characteristics of the virus,so as to provide scientific basis for the prevention and control of HFRS in Hubei Province.Methods Tissue RNA was extracted from rat lung samples by viral DNA/RNA extraction kit,and nested PCR amplification was performed.Agarose electrophoresis analysis and gene sequencing were performed.The sequence obtained was compared with the gene sequences of previous strains by DNAstar seqman software,and homology analysis was performed by MegAlign software.Phylogenetic tree was constructed by MEGA11.0 software Neighbor-Joining Method.Results A total of 1603 host animals were captured in this survey.The dominant host animals were mainly Apodemus agrarius(663,41.36%,663/1603),followed by Rattus flavithorax(359,22.40%,359/1603)and Rattus norvegicus(359,10.42%,167/1603).A total of 104 hantavirus positive mice were detected,and the overall virus carrying rate was 6.49%.The positive mice were mainly Apodemaagrarius and Rattus norvegicus,accounting for 44.23%(46/104)and 20.19%(21/104),respectively.The molecular evolution analysis showed that 42 HTNV virus samples detected in Hubei province in 2022 were highly homologous to HV004 virus strain in Hubei province,and 12 SEOV virus strains and WuhanRf02 belonged to the same branch and subbranch,and did not show obvious variation.Conclusion The results of rodent infection showed that Apodemus agrarius and Rattus norvegicus had a high rate of virus infection in Hubei Province,suggesting that there was a risk of infection in this area.The molecular evolution of the virus is stable,and no obvious mutation has occurred.At the same time,virus surveillance should be further strengthened.