白纹伊蚊细胞色素P450 CYP4H42v2蛋白生物信息学分析

Bioinformatics analysis of the Cytochrome P450CYP4H42v2 protein in Aedes albopictus

目的运用生物信息学方法预测分析白纹伊蚊细胞色素CYP4H42v2蛋白的结构与功能。方法基于白纹伊蚊基因组数据库筛选出CYP4H42v2基因并进行PCR验证;利用在线软件Expasy分析该基因编码的CYP4H42v2蛋白的理化性质和亲疏水性;利用HMMTOP version 2.0和SignalP 4.1 server分别预测CYP4H42v2蛋白的跨膜结构域以及信号肽;运用NetPhos、NCBI-Conserved domains、SOPMA、SWISS-MODEL、IEDB在线程序分别预测CYP4H42v2蛋白潜在的磷酸化位点、保守域结构、蛋白二级、三级结构及其抗原表位;对其编码的氨基酸序列进行同源比对,利用MEGA X软件构建系统进化树,通过STRING数据库对其蛋白互作网络进行预测。结果CYP4H42v2基因全长1664 bp,开放阅读框为1508 bp,编码502个氨基酸;无信号肽,存在跨膜结构域,为疏水性蛋白,有潜在的磷酸化位点和保守域结构;CYP4H42v2蛋白具有α螺旋、β折叠结构和互变异构酶活性;CYP4H42v2蛋白具有18个抗原表位,在STRING数据库共鉴定出11个相关基因以及43个互作关系。结论利用生物信息学方法预测分析白纹伊蚊CYP4H42v2基因,发现其编码蛋白存在潜在抗原表位,且具有多个磷酸化位点,为CYP4基因在白纹伊蚊抗药性机制的研究中发挥的作用提供理论基础。

Objective To predicted the structure and function of Aedes albopictus Cytochrome CYP4H42v2 proteinby bioinformatics.Methods The CYP4H42v2 gene was screened from the A.albopictus genome database and subjected to confirm by using a PCR assay.Expasy was used to analyze the physicochemical properties and hydrophobicity of the gene encoding the A.albopictus CYP4H42v2 protein.HMMTOP version 2.0 and SignalP 4.1 server were used to predict transmembrane domain and Signal peptide of the A.albopictus CYP4H42v2 protein.NetPhos,NCBI-Conserved Domains,SOPMA,Swiss-Model and IEDB Online program were used to predict the potential phosphorylation site,Conserved domain structure,protein structure and antigenic epitopes of the A.albopictus CYP4H42v2 protein.Homology analysis of the amino acid sequences was performed,and the phylogenetic tree was built by the MEGA X software.In addition,the protein-protein interaction network was deduced from the STRING database.Results The entire length of the A.albopictus CYP4H42v2 gene in italics is 1664 bp,with an open reading frame of 1508 bp,encoding 502 amino acids.A.albopictus CYP4H42v2 has no signal peptide but exists transmembranedomain,the protein is hydrophilic and has potential phosphorylation sites and conserved domain structure,αhelix,βfolding structure and tautomerase activity.The CYP4H42v2 protein has 18 antigenic epitopes,and a total of 11 related genes and 43 interaction relationships have been identified in the STRING database.Conclusion Bioinformatics predicts that the CYP4H42v2 gene of A.albopictus has potential antigenic epitope regions and multiple phosphorylation sites,providing a theoretical basis for the role of CYP4 in the resistance mechanism of A.albopictus.