miR-145、miR-186表达与非小细胞肺癌组织临床病理特征和PI3K/Akt/mTOR信号通路的关系

Relationship between the Expression of miR-145 and miR-186 and Clinical Pathological Characteristics and PI3K/Akt/mTOR Signaling Pathway in Non-Small Cell Lung Cancer Tissues

目的:探讨微小核糖核酸(miR)-145、miR-186表达与非小细胞肺癌(NSCLC)临床病理特征和磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)/哺乳动物雷帕霉素靶蛋白(mTOR)信号通路的关系。方法:选择2020年3月至2023年3月我院收治的128例行肺癌根治手术治疗的NSCLC患者,取其术中癌组织和癌旁组织(距离癌组织5 cm以上),采用实时荧光定量聚合酶链式反应(qRT-PCR)检测miR-145、miR-186以及PI3K mRNA、Akt mRNA、mTOR mRNA表达。分析miR-145、miR-186表达与NSCLC患者临床病理特征的关系;Pearson检验分析NSCLC患者癌组织miR-145、miR-186表达与PI3K mRNA、Akt mRNA、mTOR mRNA表达的相关性。结果:癌组织miR-145、miR-186表达低于癌旁组织(P<0.05),低分化、TNMⅢA期、淋巴结转移的NSCLC组织miR-145、miR-186表达低于中高分化、TNMⅠ~Ⅱ期、未发生淋巴结转移的NSCLC组织(P<0.05)。癌组织PI3K mRNA、Akt mRNA、mTOR mRNA表达均高于癌旁组织(P<0.05),癌组织miR-145、miR-186表达与PI3K mRNA、Akt mRNA、mTOR mRNA表达均呈负相关(P<0.05)。结论:NSCLC组织中miR-145、miR-186表达下调,miR-145、miR-186表达下调可能激活PI3K/Akt/mTOR信号通路促使NSCLC进展,且与NSCLC患者癌组织低分化、TNMⅢA期、淋巴结转移有关。

Objective:To investigate the relationship between the expression of micro ribonucleic acid(miRNA)-145,miR-186 and clinical pathological characteristics and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway in non-small cell lung cancer(NSCLC)tissues.Methods:128 NSCLC patients who underwent radical surgery for lung cancer in our hospital From March 2020 to March 2023 were selected,the intraoperative cancer tissues and cancer adjacent tissues(more than 5cm from the cancer tissues)were taken,the expression of miR-145,miR-186,PI3K mRNA,Akt mRNA and mTOR mRNA was detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The relationship between the expression of miR-145 and miR-186 and the clinical pathological characteristics of NSCLC patients was analyzed.The correlation between the expression of miR-145,miR-186 and the expression of PI3K mRNA,Akt mRNA and mTOR mRNA in NSCLC patients were analyzed by Pearson test.Results:The expression of miR-145 and miR-186 in cancer tissues was lower than that in cancer adjacent tissues(P<0.05),the expression of miR-145 and miR-186 in NSCLC tissues with low differentiation,TNM IIIA stage and lymph node metastasis was lower than that in NSCLC tissues with medium to high differentiation,TNM I~II stage and without lymph node metastasis(P<0.05).The expression of PI3K mRNA,Akt mRNA and mTOR mRNA in cancer tissues was higher than that in cancer adjacent tissues(P<0.05),the expression of miR-145 and miR-186 in cancer tissues was negatively correlated with the expression of PI3K mRNA,Akt mRNA and mTOR mRNA(P<0.05).Conclusion:The expression of miR-145 and miR-186 in NSCLC tissues is down-regulate,the down-regulate of miR-145 and miR-186 expression may activate the PI3K/Akt/mTOR signaling pathway to promote the progression of NSCLC,and is associate with poor differentiation of cancer tissue,TNM IIIA stage and lymph node metastasis in NSCLC patients.