血清微小RNA-141-3p、微小RNA-150-5p与鼻咽癌患者临床病理特征和放疗敏感性的关系研究

Study on the Relationship between Serum microRNA-141-3p,microRNA-150-5p and Clinical Pathological Characteristics and Radiotherapy Sensitivity in Patients with Nasopharyngeal Carcinoma

目的:探讨血清微小核糖核酸(miRNA)-141-3p、miR-150-5p与鼻咽癌(NPC)患者临床病理特征和放疗敏感性的关系。方法:收集2019年1月~2022年7月在我院接受放疗的92例NPC患者为NPC组,根据放疗疗效分为抵抗组和敏感组,另选取同期80名在我院体检的健康志愿者为对照组。比较对照组、NPC组血清miR-141-3p、miR-150-5p表达。分析NPC患者血清miR-141-3p、miR-150-5p表达与临床病理特征的关系。利用单因素和多因素Logistic回归分析NPC患者放疗抵抗的影响因素。结果:与对照组比较,NPC组血清miR-141-3p表达升高,miR-150-5p表达降低(P<0.05)。NPC患者血清miR-141-3p、miR-150-5p表达在不同分化程度、TNM分期和淋巴结转移中比较有差异(P<0.05)。92例NPC患者放疗抵抗发生率为22.83%(21/92)。单因素分析显示,抵抗组TNM分期Ⅲ~Ⅳa期和miR-141-3p≥2.60比例高于敏感组,miR-150-5p≥0.80比例低于敏感组(P<0.05)。多因素Logistic回归分析显示,miR-141-3p≥2.60为NPC患者放疗抵抗的独立危险因素,miR-150-5p≥0.80为独立保护因素(P<0.05)。结论:NPC患者血清miR-141-3p高表达,miR-150-5p低表达,与分化程度、TNM分期、淋巴结转移和放疗敏感性有关,有望成为NPC患者放疗抵抗的评价指标。

Objective:To explore the relationship between serum microribonucleic acid(miRNA)-141-3p,miR-150-5p and clinical pathological characteristics and radiotherapy sensitivity in patients with nasopharyngeal carcinoma(NPC).Methods:92 NPC patients with who were underwent radiotherapy in our hospital from January 2019 to July 2022 were collected as NPC group,they were divided into resistance group and sensitivity group based on the efficacy of radiotherapy,and another 80 healthy volunteers who were underwent physical examination in our hospital during the same period were selected as control group.The expression of serum miR-141-3p and miR-150-5p between control group and NPC group were compared.The relationship between the expression of serum miR-141-3p,miR-150-5p and clinical pathological characteristics in patients with NPC was analyzed.The influencing factors of radiotherapy resistance in patients with NPC was analyzed by univariate and multivariate Logistic regression.Results:Compared with control group,the expression of serum miR-141-3p increased in NPC group,and the expression of miR-150-5p decreased(P<0.05).The expression of serum miR-141-3p and miR-150-5p in patients with NPC showed significant differences in different degrees of differentiation,TNM stage,and lymph node metastasis(P<0.05).The incidence of radiation resistance in 92 patients with NPC was 22.83%(21/92).Univariate analysis showed that the proportion of TNM stage III-IVa and miR-141-3p≥2.60 were higher in the resistant group than in the sensitive group,and the proportion of miR-150-5p≥0.80 was lower than in the sensitive group(P<0.05).Multivariate Logistic regression analysis showed that miR-141-3p≥2.60 was the independent risk factor of radiation resistance in patients with NPC,and miR-150-5p≥0.80 was the independent protective factor(P<0.05).Conclusion:High expression of serum miR-141-3p in patients with NPC,low expression of miR-150-5p,which are related to degrees of differentiation,TNM stage,lymph node metastasis,and radiotherapy sensitivity,and which are expected to become the evaluation indicators of radiotherapy resistance in NPC patients.