寿司中菌落总数检测的不确定度评定

Uncertainty Evaluation of Total Colony Count Testing in Sushi

目的分析20份市售寿司菌落总数检验结果测量不确定度的来源,计算A类不确定度、B类不确定度等,评定测量不确定度,确定其置信区间。方法选取2023年4—8月20份市售寿司进行分析。按照《测量不确定度评定与表示》要求,参照《食品安全国家标准食品微生物学检验菌落总数测定》以及相关统计学方法进行计算。结果A类不确定度为0.0445,B类不确定度为0.0147,合成标准不确定度为0.0469,扩展不确定度为0.0978。结论实验室在进行微生物培养时需要一个稳定的环境,这种环境能够有效地保障检验结果的准确性和精密度,食品菌落总数的不确定度评定是确保食品安全质量的重要手段。

Objective To analyze the sources of uncertainty in measurement of the total bacterial count of 20 sushi samples,calculate the uncertainty of Class A and class B,evaluate the uncertainty of measurement,and determine the confidence interval.Methods Select 20 commercially available sushi from April to August 2023 for analysis.According to the requirements of“Evaluation and expression of uncertainty in measurement”,the food safety were calculated according to“National Standard for food safety,microbiological examination,determination of total colony count”and related statistical methods.Results The uncertainty of class A was 0.0445,class B was 0.0147,the uncertainty of synthetic standard was 0.0469,and the uncertainty of expanded was 0.0978.Conclusion The laboratory needs a stable environment to carry out microbial culture,which can effectively guarantee the accuracy and precision of test results,the uncertainty evaluation of the total number of food colony is an important means to ensure the safety and quality of food.