血清干扰素γ诱导蛋白10和甘露糖结合凝集素在再生障碍性贫血和骨髓增生异常综合征患者中的含量及临床意义

The content and clinical significance of interferonγ-induced protein 10 and mannose-binding lectin in serum of aplastic anemia and myelodysplastic syndrome patients

目的探讨血清干扰素γ诱导蛋白10(IP10)和甘露糖结合凝集素(MBL)在再生障碍性贫血(AA)和骨髓增生异常综合征(MDS)患者中的含量及临床意义。方法选取2019年1月至2021年6月在昆山市第三人民医院初诊的26例AA患者(AA组)和42例MDS患者(MDS组)作为观察组,另选取30名健康体检者作为对照组。检测并比较两组血清IP10和MBL水平,分析两组血清IP10与MBL的相关性,比较两组不同淋巴细胞亚群比例及CD4^(+)、CD8^(+)淋巴细胞比值。结果AA组和MDS组血清IP-10水平均高于对照组,血清MBL水平均低于对照组,且AA组血清IP-10水平高于MDS组,AA组血清MBL水平低于MDS组,差异有统计学意义(P<0.05)。Pearson线性相关分析结果显示,两组血清IP-10与MBL均呈负相关性(r<0,P<0.05)。AA组外周血总CD3^(+)T淋巴细胞比例高于MDS组,差异有统计学意义(P<0.05);两组外周血CD3-CD16+/CD56+NK淋巴细胞比例和CD19+B淋巴细胞比例比较差异无统计学意义。AA组CD3^(+)CD8^(+)T淋巴细胞比例高于MDS组,而CD4^(+)/CD8^(+)比值低于MDS组,差异有统计学意义(P<0.05)。结论通过检测患者血清IP-10和MBL水平,以及分析外周血T淋巴细胞亚群CD4^(+)/CD8^(+)比值,有助于为AA及MDS的发病机制、早期鉴别诊断等方面提供更有价值的信息。

Objective To investigate the content and clinical significance of interferonγ-induced protein 10(IP10)and mannose-binding lectin(MBL)in serum of aplastic anemia(AA)and myelodysplastic syndrome(MDS)patients.Methods A total of 26 patients with AA(AA group)and 42 patients with MDS(MDS group)who were newly diagnosed in the Third People's Hospital of Kunshan from January 2019 to June 2021 were se-lected as the observation group,and 30 healthy physical examination subjects were selected as the control group.The levles of IP10 and MBL were determined and compared between the two groups,the correlation between serum IP10 and MBL were analyzed in the two groups,the proportions of different lymphocyte subpopulations and the ratios of CD4^(+)and CD8^(+)lymphocytes were compared between the two groups.Results The serum lev-els of IP-10 in AA and MDS groups was higher than those in control group,the level of MBL was lower than that in control group,and the differenc-es were statistically significant(P<0.05).Pearson linear correlation analysis showed that there was a negative correlation between serum IP-10 and MBL of the two groups(r<0,P<0.05).The proportion of total CD3^(+)T lymphocytes in peripheral blood in the AA group was higher than that in the MDS group,and the difference was statistically significant(P<0.05);there were no significant differences in the proportion of CD3-CD16+/CD56+NK lymphocytes and CD19+B lymphocytes in peripheral blood between the two groups.The proportion of CD3^(+)CD8^(+)T lymphocytes in the AA group was higher than that in the MDS group,The ratio of CD4^(+)/CD8^(+)in the AA group was lower than that in the MDS group,the differences were statisti-cally significant(P<0.05).Conclusion Detecting the contents of IP10 and MBL in serum and analyzing the ratio of CD4^(+)/CD8^(+)in T lymphocyte subsets could be used as indicators for differential diagnosis of AA and MDS patients.