宫颈癌组织LncRNA HOTAIR、miR-17-5p表达与增殖基因、临床病理特征和预后的关系分析

Analysis of the Relationship between the Expression of LncRNA HOTAIR and miR-17-5p and Proliferation Genes,Clinicopathological Features and Prognosis in Cervical Cancer Tissue

目的:探讨宫颈癌组织长链非编码核糖核酸(LncRNA)HOX转录反义RNA(HOTAIR)、微小核糖核酸(miR)-17-5p表达与增殖基因、临床病理特征和预后的关系。方法:选择2014年1月-2018年1月新疆医科大学第一附属医院收治的157例宫颈癌患者,取手术切除的宫颈癌组织和癌旁组织,实时荧光定量聚合酶链反应(RT-PCR)法检测LncRNA HOTAIR、miR-17-5p及宫颈癌相关增殖基因PRDX4、NEK2、FHIT、BLCAP转录水平表达。Pearson分析宫颈癌组织LncRNA HOTAIR、miR-17-5p表达与增殖基因表达的相关性,Kaplan-Meier法分析不同LncRNA HOTAIR、miR-17-5p表达宫颈癌患者的5年生存率差异。结果:宫颈癌组织LncRNA HOTAIR、miR-17-5p、PRDX4、NEK2、FHIT、BLCAP转录水平表达均高于癌旁组织(P<0.05)。宫颈癌组织LncRNA HOTAIR、miR-17-5p表达与PRDX4、NEK2、FHIT、BLCAP转录水平表达均呈正相关(P<0.05),LncRNA HOTAIR表达与miR-17-5p表达呈正相关(P<0.05)。国际妇产科联盟(FIGO)分期ⅡA期、低分化、肌层浸润≥1/2、淋巴结转移癌组织LncRNA HOTAIR、miR-17-5p表达显著高于FIGO分期Ⅰ期、中高分化、肌层浸润<1/2、无淋巴结转移(P<0.05)。随访期间失访3例,存活132例。LncRNA HOTAIR高表达患者5年随访期间总生存(OS)率低于LncRNA HOTAIR低表达患者(P<0.05),miR-17-5p高表达患者5年OS率低于miR-17-5p低表达患者(P<0.05)。结论:宫颈癌组织中LncRNA HOTAIR、miR-17-5p表达均上调,且与增殖基因表达上调、低分化、高FIGO分期、较深的肌层浸润、淋巴结转移和低生存率有关。

Objective:To investigate the relationship between the expression of long chain non-coding ribonucleic acid(LncRNA)HOX transcriptional antisense RNA(HOTAIR)and microribonucleic acid(miR)-17-5p and proliferation genes,clinicopathological features and prognosis in cervical cancer tissues.Methods:157 cervical cancer patients who were admitted to The First Affiliated Hospital of Xinjiang Medical University from January 2014 to January 2018 were selected,cervical cancer tissues and cancer adjacent tissues were collected,the expression levels of LncRNA HOTAIR,miR-17-5p and cervical cancer-related proliferation genes PRDX4,NEK2,FHIT and BLCAP were detected by real-time fluorescence quantitative polymerase chain reaction(RT-PCR).The correlation between the expression of LncRNA HOTAIR,miR-17-5p and the expression of proliferation genes in cervical cancer tissues were analyzed by Pearson,the difference in 5-year survival rate of cervical cancer patients with different LncRNA HOTAIR and miR-17-5p expression were analyzed by Kaplan-Meier method.Results:The transcriptional levels of LncRNA HOTAIR,miR-17-5p,PRDX4,NEK2,FHIT and BLCAP in cervical cancer tissues were higher than those in cancer adjacent tissues(P<0.05).The expression of LncRNA HOTAIR and miR-17-5p in cervical cancer tissues was positively correlated with the transcription levels of PRDX4,NEK2,FHIT and BLCAP(P<0.05),the expression of LncRNA HOTAIR was positively correlated with the expression of miR-17-5p(P<0.05).The expression of LncRNA HOTAIR and miR-17-5p in cancer tissues of International Federation of Gynecology and Obstetrics(FIGO)stage IIA,low differentiation,muscular invasion≥1/2 and lymph node metastasis was significantly higher than that of FIGO stage I,moderate to high differentiation,muscular invasion<1/2 and no lymph node metastasis(P<0.05).3 cases were lost during the follow-up period,and 132 cases survived.The 5-year overall survival(OS)rate of patients with high expression of LncRNA HOTAIR was lower than that of patients with low expression of LncRNA HOTAIR(P<0.05),and the 5-year OS rate of patients with high expression of miR-17-5p was lower than that of patients with low expression of miR-17-5p(P<0.05).Conclusion:The expression of LncRNA HOTAIR and miR-17-5p in cervical cancer tissues are up-regulate,which are relate to the up-regulation of proliferation gene expression,poor differentiation,high FIGO stage,deep myometrial invasion,lymph node metastasis and low survival rate.