西藏胡黄连中1个新的葫芦烷型糖苷化合物

A new cucurbitane glycoside from Picrorhiza scrophulariiflora

采用柱色谱方法对西藏胡黄连Picrorhiza scrophulariiflora乙醇提取物进行提取分离,并通过HR-MS、1D和2D NMR等技术鉴定结构,同时采用CCK-8法检测细胞毒性。结果分离得到4个葫芦烷型糖苷,分别为2β-D-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5,25-二烯-22-酮(1)、2β-D-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5,24-二烯-22-酮(2)、25-乙酰氧基-2β-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5-烯-22-酮(3)、25-乙酰氧基-2β-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5,23-(E)-二烯-22-酮(4),其中化合物1为1个新的葫芦烷型糖苷。对4种肿瘤细胞的半数抑制浓度均大于100μmol·L^(-1),表明这4个化合物无明显细胞毒作用。

Chemical constituents from the ethanol extract of Picrorhiza scrophulariiflora were isolated and purified by column chromatography.Their structures were identified by HR-MS,1D and 2D-NMR,and their cytotoxicity was assessed by CCK-8 assay.Four compounds were isolated and identified as follows:2β-D-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosterol-5,25-diene-22-one(1),2β-D-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5,24-diene-22-one(2),25-acetoxy-2β-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5-ene-22-one(3)and 25-acetoxy-2β-glucosyloxy-3β,16α,20β-trihydroxy-9-methyl-19-norlanosta-5,23-(E)-diene-22-one(4).Compound 1 represents a new cucurbitane glycoside.The half inhibitory concentrations of the 4 compounds exceeded 100μmol·L^(-1)against four tumor cell lines,indicating no significant cytotoxicity.