硫素水平对大豆叶片碳氮代谢关键酶活性的影响

Effects of Sulfur Level on Key Enzymes Activities in Carbon and Nitrogen Metabolism in Soybean Leaves

为了探究不同的硫素水平对大豆苗期和花期叶片碳氮代谢关键酶活性的影响,以大豆品种‘来豆2号’和‘合丰55号’为试材,用浇灌营养液的方法测大豆苗期和花期叶片碳代谢关键酶活性的数据。‘来豆2号’和‘合丰55号’的GOGAT和SS酶活性在160 mg/L处理下达最大值;‘来豆2号’GS酶活性在160 mg/L处理下酶活性峰值最强,‘合丰55号’苗期峰值是260 mg/L处理下达最大值,花期峰值是硫营养最低的20 mg/L处理下;‘来豆2号’SPS酶活性在40 mg/L处理下达最大值,而‘合丰55号’苗期峰值是硫营养较高的80 mg/L处理,花期峰值是硫素水平最高的260 mg/L处理。结果表明,谷氨酸合成酶(GOGAT)、谷氨酰胺合成酶(GS)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)的开花期酶活性显著大于苗期,品种间呈相同规律。

In order to explore the effects of different sulfur levels on the key enzyme activities in carbon and nitrogen metabolism in soybean leaves at seedling and flowering stages,soybean varieties‘Laidou 2’and‘Hefeng 55’were used as test materials.The data of key enzyme activities of carbon metabolism in soybean leaves at seedling and flowering stages were measured by watering nutrient solution.The GOGAT and SS enzyme activities of‘Laidou 2’and‘Hefeng 55’reached the maximum under 160 mg/L treatment.The peak value of GS enzyme activity of‘Laidou 2’was the strongest under 160 mg/L treatment,the peak value of‘Hefeng 55’in seedling stage was the highest under 260 mg/L treatment,and the peak value in flowering stage was the lowest under 20 mg/L treatment of sulfur nutrition.The SPS enzyme activity of‘Laidou 2’reached the maximum value under 40 mg/L treatment,while the peak value of‘Hefeng 55’at seedling stage was under 80 mg/L treatment with higher sulfur nutrition,and the peak value at flowering stage was under 260 mg/L treatment with the highest sulfur level.The results showed that the activities of glutamate synthase(GOGAT),glutamine synthetase(GS),sucrose phosphate synthase(SPS)and sucrose synthase(SS)at flowering stage were significantly higher than those at seedling stage,and the same rule was found among varieties.