激素配比对甘薯组织培养及试管苗移栽后生长的影响

Effects of Hormone Proportions on Tissue Culture of Sweet Potato and Tube Seedlings Growth After Transplantation

研究植物外源激素6-BA和NAA不同配比对甘薯愈伤分化、继代扩繁、试管苗移栽后生长的影响,建立高效的甘薯茎尖离体脱毒培养和快速繁殖技术体系。以推广应用面积较大的甘薯品种‘烟薯25’、‘普薯32’、‘龙薯9号’和‘商薯19’为试验材料,在MS培养基中添加6个不同浓度配比的6-BA、NAA,配制愈伤分化培养基和继代扩繁培养基。探讨茎尖组织愈伤率、成苗率、愈伤组织直径、叶片数,同时调查试管苗移栽成活率、叶片数量、株高、基部茎粗等的差异,研究组培苗愈伤分化、继代扩繁和移栽后生长的最佳配方。‘烟薯25’和‘普薯32’的愈伤分化成苗率在A4处理(1.5 mg/L 6-BA+0.2 mg/L NAA)下最高,‘龙薯9号’和‘商薯19’愈伤分化成苗率在A3处理(1.5 mg/L 6-BA+0.1 mg/L NAA)下最高;‘烟薯25’在B4处理(0.5 mg/L 6-BA+0.4 mg/L NAA)下继代扩繁培养长势较好,移栽后成活率高、生长快,‘普薯32’、‘龙薯9号’和‘商薯19’在B3处理(0.5 mg/L 6-BA+0.2 mg/L NAA)下继代扩繁培养长势较好、移栽后成活率最高、生长快。在甘薯茎尖组织愈伤分化、继代扩繁过程中,不同甘薯品种适宜的6-BA、NAA配比存在显著差异,实际生产过程中应充分考虑品种特性。

The paper aims to study the effects of different ratios of 6-BA and NAA on sweet potato callus differentiation,subculture multiplication and growth of test-tube seedlings after transplanting,and to establish an efficient system for virus-free in vitro tip meristem culture and rapid propagation technology.Four widely cultivated sweet potato cultivars,‘Yanshu 25’,‘Pushu 32’,‘Longshu 9’and‘Shangshu 19’,were used for this experiment.Six 6-BA/NAA concentration ratios were added to MS medium to prepare the induction differentiation medium and the subculture multiplication medium.The callus differentiation rate of tip meristem,seedling formation rate,diameter of callus tissue and number of leaves were investigated,and the differences in survival rate of test-tube plantlets after transplanting,number of leaves,plant height and basal stem thickness were investigated as well to explore the optimal formulations for the callus differentiation,subculture multiplication,and the growth of test-tube seedlings after transplanting.The highest callus differentiation rate of‘Yanshu 25’and‘Pushu 32’was obtained at A4 treatment(1.5 mg/L 6-BA+0.2 mg/L NAA),and the highest callus differentiation rate of‘Longshu 9’and‘Shangshu 19’was obtained at A3 treatment(1.5 mg/L 6-BA+0.2 mg/L NAA).‘Yanshu 25’showed better growth in subculture multiplication culture,had a higher survival rate and grew faster in B4 treatment(0.5 mg/L 6-BA+0.4 mg/L NAA)than others.The optimal medium for‘Pushu 32’,‘Longshu 9’and‘Shangshu 19’was determined as B3 treatment(0.5 mg/L 6-BA+0.2 mg/L NAA),with the best growth in subculture multiplication culture,the highest survival rate and the fastest growth after transplanting.In the process of sweet potato callus differentiation and rapid propagation,there were significant differences in the appropriate proportion of 6-BA and NAA for different sweet potato cultivars,and the cultivars characteristics should be fully considered in the actual production.