低磷胁迫大豆CHX基因家族生物信息学分析

Bioinformatics analysis of CHX gene family in soybean under low phosphorus stress

【目的】探究大豆耐低磷的分子机制,并分析大豆CHX基因编码的相应蛋白质功能及性质。【方法】比较近等基因系(NIL6)高磷和低磷水平下氢离子分泌量,然后进行全基因组重测序,通过基因注释筛选阳离子质子转运体(CHX)家族基因,分析CHX家族基因及其调控蛋白基本理化性质、染色体定位等信息。【结果】低磷条件下,NIL6根系泌氢能力显著高于高磷水平,泌氢增加3.76倍。通过对NIL6重测序进行基因注释,筛选得到21个有关CHX基因,对其结构域分析,确定基因属于CHX基因家族,对CHX家族基因生物信息学分析发现编码蛋白的氨基酸数目为505~914,分子量介于55.38~100.61 kD,等电点介于5.53~9.26,发现其调控的蛋白质阿尔法螺旋占比都在50%以上;通过亚细胞定位预测显示大豆CHX家族基因编码的蛋白质在细胞膜定位数量为21个,内质网中的定位数量为16个,基因编码蛋白亲水性均大于0;通过系统发育进化树分析,发现每个亚组的成员之间亲缘关系较近;通过保守序列基序分析,发现聚类关系较近的基因所包含的Motif数量相似;通过染色体定位可知,CHX家族基因绝大多数远离中心粒,定位于各个染色体两端。CHX家族蛋白均主要由α-螺旋组成,为不亲水性蛋白编码,主要在细胞膜和内质网中发挥作用。【结论】大豆CHX基因家族在维持细胞pH稳定和细胞生长中起重要作用,可能参与植物逆境胁迫等生长过程,染色体定位结果表明CHX基因家族在大豆自然生长发育过程中存在遗传变异。

【Objective】The article aims to explore the molecular mechanism of soybean's tolerance to low phosphorus and analyze the functions and properties of the corresponding proteins encoded by soybean CHX genes.【Method】The study compared hydrogen ion secretion at high and low phosphorus levels in a near-isogenic line(NIL6),then performed whole-genome resequencing,screened cationic proton transporter(CHX)family genes by gene annotation,and analyzed information on CHX family genes and their basic physicochemical properties and chromosomal localization of regulatory proteins.【Result】Under the condition of low phosphorus regulation,the hydrogen secretion ability of NIL6 roots was significantly higher than that of its high phosphorus level,and the hydrogen secretion increased by 3.76 times.Through gene annotation of NIL6 re-sequence,21 CHX-related genes were screened,and their domain analysis confirmed that the genes belonged to the CHX gene family.Bioinformatics analysis of the CHX family gene showed that the number of amino acids encoding the protein was between 505-914,the molecular weight was between 55.38-100.61 kD,and the isoelectric point was between 5.53-9.26.It was found that the proportion of alpha helices of the protein regulated by them was more than 50%.The subcellular localization prediction showed that the number of proteins encoded by soybean CHX family genes was 21 in the cell membrane,16 in the endoplasmic reticulum,and the hydrophilicity of gene encoded proteins was greater than 0.Through phylogenetic evolution tree analysis,it was found that the members of each subgroup were relatively close to each other;Through conservative sequence analysis,it was found that the number of Motif contained in genes with close clustering relationship was similar;The majority of CHX family genes were located at both ends of each chromosome,far from the centromere.Bioinformatics analysis showed that all the proteins of this family consisted mainly ofα-helices,and the proteins encoded by soybean CHX family genes were mainly non-hydrophilic proteins encoded mainly in the cell membrane and endoplasmic reticulum.【Conclusion】The soybean CHX gene family plays an important role in maintaining cell pH stability and cell growth,and might be involved in plant growth process under stress.The results of chromosome mapping indicate that CHX gene family has genetic variation during the natural growth and development of soybean.