栀子WRKY基因家族鉴定及其响应盐胁迫的表达模式

Identification of the WRKY gene family in Gardenia jasminoides and its expression patterns in response to salt stress

【目的】为进一步研究WRKY基因家族在调控栀子盐胁迫中的功能作用、培育栀子抗盐新品种。【方法】利用生物信息学方法对栀子WRKY基因家族成员(GjWRKY)进行全基因组鉴定和相关分析。【结果】共鉴定出47个WRKY基因,非均匀地分布在10条染色体上,通过系统发育树将其分为3大组:GroupⅠ、Ⅱ和Ⅲ。GjWRKY基因以串联重复为主要扩增方式,且多数基因成员含有3个外显子。GjWRKY基因家族启动子2000 bp区域含有大量激素类和胁迫类响应顺式作用元件。46个GjWRKY基因在中度(MS)和重度盐胁迫(SS)下具有不同程度的高表达,部分GjWRKY基因产生特异性表达,17个GjWRKY基因表现为上调表达,推测这部分GjWRKY基因可能起正调控作用。【结论】GjWRKY基因在栀子抗盐胁迫中发挥了重要作用。

【Objective】To further investigate the functional role of the WRKY gene family in regulating salt stress in Gardenia jasminoides and cultivate new salt resistant varieties.【Method】The WRKY gene family members of G.jasminoides(GjWRKY)were identified and analyzed by bioinformatics.【Result】The study identified a total of 47 WRKY genes,which were unevenly distributed on 10 chromosomes.They were divided into Group I,II and III groups by phylogenetic tree.The GjWRKY gene family was mainly amplified by tandem repeat,and most members of the gene family contain three exons.The 2000 bp region of the GjWRKY gene family promoter contained a large number of hormone and stress responsive cis-acting elements.46 GjWRKY genes have different degrees of high expression under moderate(MS)and severe salt stress(SS),and some GjWRKY genes have specific expression,17 GjWRKY genes expression were up-regulated,suggesting that these GjWRKY genes may play a positive role in regulation.【Conclusion】The above indicates that the GjWRKY genes plays an important role in the salt stress resistance of G.jasminoides.