COL1A1和COL1A2基因生信分析及其在梅花鹿不同组织中的表达谱

Bioinformatics analysis of COL1A1 and COL1A2 genes and their expression profiles in different tissues of sika deer

【目的】探明COL1A1(Collagen type I alpha 1 chain,I型胶原蛋白α1链)和COL1A2(Collagen type I alpha 2 chain,I型胶原蛋白α2链)基因在梅花鹿不同组织中的表达谱,解析其对梅花鹿组织发育的影响,为影响梅花鹿重要经济性状的候选基因筛选提供依据。【方法】采用RT-qPCR方法检测COL1A1和COL1A2基因在雄性梅花鹿心脏、肝脏和脾脏等16个组织器官中的表达水平;结合NetPhos 3.1、Motif Search和ProtParam等系列软件预测分析COL1A1和COL1A2基因的生物信息及其在梅花鹿不同组织中的表达谱,并在此基础上构建COL1A1和COL1A2氨基酸序列的系统发育进化树。【结果】COL1A1和COL1A2基因CDS区分别编码1463和1364个氨基酸,理论PI分别为5.60和9.19,COL1A1和COL1A2均是一种具有信号肽和磷酸化位点的亲水性稳定蛋白质;二者蛋白二级及三级结构均以无规则卷曲构成;与其他动物相比,鹿COL1A1和COL1A2基因均与反刍动物山羊、绵羊和牛的同源性最高,其中,鹿COL1A1基因与山羊、牛、绵羊的同源性分别为99.5%、99.5%和99.2%,鹿COL1A2基因与牛、绵羊、山羊的同源性分别为99.1%、99.0%和98.9%,亲缘关系最近。RT-qPCR结果显示,COL1A1和COL1A2基因在梅花鹿不同组织中均有表达,其中COL1A1基因在心脏、背最长肌和腿肌中的表达较高,显著高于其他组织,COL1A2基因在心脏、肝脏、肾脏和瓣胃中的表达较高,均显著高于其他组织;此外,COL1A1在肌肉组织中的表达较高,COL1A2较低;二者在其余组织中的表达具有一高一低,相互协同的作用趋势。【结论】COL1A1和COL1A2可能通过相互协同共同维持组织结构及组织发育,相关结果为后续深入研究COL1A1和COL1A2影响梅花鹿生长发育奠定基础。

【Objective】The present paper aimed to explore the expression profiles of COL1A1(Collagen type I alpha 1 chain)and COL1A2(Collagen type I alpha 2 chain)genes in different tissues of sika deer,analyze their effects on tissue development of sika deer,and provide a basis for selecting candidate genes affecting important economic traits of sika deer.【Method】The expression levels of COL1A1 and COL1A2 genes in heart,liver,spleen,lung,kidney,large intestine,small intestine,cecum,duodenum,rumen,reticulum,omasum,abomasum,longissimus dorsi,leg muscle and testis of male sika deer were detected by RT-qPCR.The biological information of COL1A1 and COL1A2 genes and their expression profiles in different tissues of sika deer were predicted and analyzed by NetPhos 3.1,Motif Search and ProtParam software.On the basis,the phylogenetic tree of COL1A1 and COL1A2 amino acid sequences was constructed.【Result】CDS regions of COL1A1 and COL1A2 genes encoded 1463 and 1364 amino acids,respectively,and the theoretical PI were 5.60 and 9.19,respectively.Both COL1A1 and COL1A2 were hydrophilic and stable proteins with signal peptides and phosphorylation sites.The secondary and tertiary structures of COL1A1 and COL1A2 proteins were composed of random coils.Compared with other animals,deer COL1A1 and COL1A2 genes had the highest homology and the closest relationship with ruminant goats,sheep and cattle.The homology of COL1A1 of deer with goats,cattle and sheep was 99.5%,99.5%and 99.2%,respectively.The homology of COL1A2 of deer with goats,cattle and sheep was 99.1%,99.0%and 98.9%,respectively.The results of RT-qPCR showed that COL1A1 and COL1A2 genes were expressed in different tissues of sika deer.The expression of COL1A1 gene was higher in heart,longissimus dorsi and leg muscle,which was significantly higher than that in other tissues,while the expression of COL1A2 gene was higher in heart,liver,kidney and omasum,which was significantly higher than that in other tissues.In addition,the expression of COL1A1 was higher in muscle tissue,while the expression of COL1A2 was lower.The expression of COL1A1 and COL1A2 in other tissues had a trend of high and low and synergistic effect.【Conclusion】COL1A1 and COL1A2 may cooperatively maintain tissue structure and tissue development,which lays a foundation for further research on the effects of COL1A1 and COL1A2 on growth and development of sika deer.