下咽鳞状细胞癌组织LncRNA SNHG1、miR-143-3p表达与临床病理特征和预后的关系

The relationship between the expression of LncRNA SNHG1 and miR-143-3p in nasopharyngeal squamous cell carcinoma tissue and clinical pathological features and prognosis

目的探讨下咽鳞状细胞癌(HSCC)组织长链非编码RNA(LncRNA)小核仁RNA宿主基因1(LncRNA SNHG1)、微小RNA(miR)-143-3p表达与临床病理特征和预后的关系。方法前瞻性选择2016年3月至2018年3月邯郸市中心医院收治的97例HSCC患者,取手术切除的HSCC组织和癌旁正常黏膜组织,采用实时荧光定量聚合酶链反应(qRT-PCR)检测LncRNA SNHG1、miR-143-3p表达,出院后随访患者生存情况。比较不同临床病理参数HSCC组织中LncRNA SNHG1、miR-143-3p表达的差异,分析LncRNA SNHG1与miR-143-3p表达的相关性以及LncRNA SNHG1、miR-143-3p表达与HSCC患者预后的关系。结果HSCC组织中LncRNA SNHG1表达高于癌旁正常黏膜组织(P<0.05),miR-143-3p表达低于癌旁正常黏膜组织(P<0.05)。TNM分期Ⅲ期、低中分化、淋巴结转移HSCC患者癌组织中LncRNA SNHG1表达高于TNM分期Ⅰ~Ⅱ期、高分化、无淋巴结转移HSCC患者(均P<0.05),miR-143-3p表达低于TNM分期Ⅰ~Ⅱ期、高分化、无淋巴结转移HSCC患者(均P<0.05)。HSCC组织中LncRNA SNHG1表达与miR-143-3p表达呈负相关(r=-0.522,P<0.05)。LncRNA SNHG1高表达HSCC患者5年累积生存率低于LncRNA SNHG1低表达HSCC患者(P<0.05),miR-143-3p低表达HSCC患者5年累积生存率低于miR-143-3p高表达HSCC患者(P<0.05)。多因素Cox回归分析显示:TNM分期Ⅲ期、高表达LncRNA SNHG1是HSCC患者预后不良的危险因素(均P<0.05),高表达miR-143-3p是其保护因素(P<0.05)。结论HSCC组织中LncRNA SNHG1表达上调,miR-143-3p表达下调,两者表达呈负相关,与HSCC恶性病理特征以及不良预后有关。

Objective To explore the relationship between the expression of long chain non coding ribonucleic acid(LncRNA)small nucleolar RNA host gene 1(LncRNA SNHG1)and microRNA(miR)-143-3p in nasopharyngeal squamous cell carcinoma(HSCC)tissue and clinical pathological features and prognosis.Methods A prospective selection was made from 97 HSCC patients admitted to the Handan Central Hospital from March 2016 to March 2018.Surgical resection of HSCC tissue and normal mucosa tissue adjacent to cancer were taken,and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of LncRNA SNHG1 and miR-143-3p.The patient's survival status was followed up after leaving the hospital.We compared the differences in the expression of LncRNA SNlHG1 and miR-143-3p in HSCC tissues with different clinical pathological parameters,analyzed the correlation between LncRNA SNHG1 and miR-143-3p expression,and the relationship between LncRNA SNHG1 and miR-143-3p expression and the prognosis of HSCC patients.Results The expression of LncRNA SNHG1 in HSCC tissue was higher than that in normal mucosa tissue adjacent to cancer(P<0.05),and the expression of miR-143-3p was lower than that in normal mucosa tissue adjacent to cancer(P<0.05).The expression of LncRNA SNHG1 in cancer tissues of HSCC patients with tumor node metastasis(TNM)stage Ⅲ,low to medium differentiation,and lymph node metastasis was higher than that of HSCC patients with TNM stage Ⅰ-Ⅱ,high differentiation,and no lymph node metastasis(all P<0.05),and the expression of miR-143-3p was lower than that of HSCC patients with TNM stage Ⅰ-Ⅱ,high differentiation,and no lymph node metastasis(all P<0.05).The expression of LncRNA SNHG1 in HSCC tissues is negatively correlated with the expression of miR-143-3p(r=-0.522,P<0.05).The 5-year cumulative survival rate of HSCC patients with high expression of LncRNA SNHG1 was lower than that of HSCC patients with low expression of LncRNA SNHG1(P<0.05),and the 5-year cumulative survival rate of HSCC patients with low expression of miR-143-3p was lower than that of HSCC patients with high expression of miR-143-3p(P<0.05).Multivariate Cox regression analysis showed that TNM stage Ⅲ and high expression of LncRNA SNHG1 were risk factors for poor prognosis in HSCC patients(all P<0.05),while high expression of miR-143-3p was a protective factor(P<0.05).Conclusions The expression of LncRNA SNHG1 is upregulated and miR-143-3p is downregulated in HSCC tissues,with a negative correlation between the two,which is related to the malignant pathological characteristics and poor prognosis of HSCC.