摘要
灰树花多肽-锌螯合物(Grifola frondosa Polypeptide Zinc Chelate,GPs-Zn)是灰树花多肽(Grifola frondosa Polypeptide,GPs)和锌离子的螯合产物,利用电镜扫描、X-射线粉末衍射技术、红外光谱和紫外光谱等技术对GPs-Zn进行结构分析;通过构建ICR母鼠缺锌模型,进一步探究GPs-Zn对孕期缺锌鼠后代的影响。表征结果表明,GPs和锌离子螯合成一种新的物质;红外光谱对比图发现肽链上的氨基和羧基都参与了锌离子的配位反应;紫外光谱图发现,GPs-Zn中多肽的羰基原子和锌离子结合,诱导紫外光谱的红移,表明GPs与锌离子结合形成GPs-Zn。动物实验表明:GPs-Zn可将缺锌仔鼠胸腺指数提高78.69%(雌鼠)和87.55%(雄鼠);脾脏指数提高40.28%(雌鼠)和43.22%(雄鼠);体质量提高89.98%(雌鼠)和88.30%(雄鼠);且血清的超氧化物歧化酶(SOD)活力提高了108.07%(雌鼠)和26.16%(雄鼠);锌浓度提高14.74%(雌鼠)和29.33%(雄鼠);碱性磷酸酶(AKP)水平降低52.28%(雌鼠)和62.48%(雄鼠)。综上可知,GPs-Zn对孕期缺锌鼠后代仔鼠的胸腺指数、脾脏指数、超氧化物歧化酶活力、锌浓度和碱性磷酸酶水平有一定改善作用。
Grifola frondosa polypeptide-zinc chelate(GPs-Zn)is the chelation product of Grifola frondosa polypeptides(GPs)and zinc ions.GPs-Zn was subjected to structural characterization using scanning electron microscopy,X-ray powder diffraction,infrared spectroscopy,and ultraviolet spectroscopy.The effects of GPs-Zn on the offspring of zinc-deficient pregnant mice were explored by constructing an adult female ICR mouse model of zinc deficiency.Characterization results showed that GPs and zinc ions formed a novel substance through chelation;IR spectral comparison revealed that both amino and carboxyl groups on the peptide chain participated in the coordination reaction of zinc ions.The UV spectrum of GPs-Zn exhibited a red shift attributed to the binding of the carbonyl atoms of the peptides to zinc ions,indicating that GPs bound to zinc ions to form GPs-Zn.Animal experiments showed that GPs-Zn increased the thymus index of zinc-deficient offspring by 78.69%(female rats)and 87.55%(male rats),the spleen index by 40.28%(female rats)and 43.22%(male rats),the body weight by 89.98%(female rats)and 88.30%(male rats),the superoxide dismutase(SOD)activity in serum by 108.07%(female rats)and 26.16%(male rats),and the zinc concentration by 14.74%(female rats)and 29.33%(male rats),but decreased the serum alkaline phosphatase(AKP)activity by 52.28%(female rats)and 62.48%(male rats).GPs-Zn can improve the thymus index,spleen index,SOD activity,zinc concentration,and AKP activity of the offspring of zinc-deficient pregnant mice.
作者
张凤丽
李静茹
彭培植
黄文琪
赵立娜
ZHANG Fengli;LI Jingru;PENG Peizhi;HUANG Wenqi;ZHAO Lina(National Engineering Research Center of JUNCAO Technology,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《现代食品科技》
CAS
北大核心
2024年第4期18-26,共9页
Modern Food Science and Technology
基金
福建省重大专项项目(2021NZ029009)
福建省科学技术厅对外合作项目(2021I0008)
福建农林大学学科交叉融合推动菌草科学及产业高质量发展项目(XKJC-712021030)
福建农林大学科技创新专项基金项目(KFb22115XA)。
