结肠癌中ALOX12表达与临床病理特征及预后的关系分析

Analysis on the relationship of ALOX12 expression with clinicopathological features and prognosis in colon cancer

目的:探讨花生四烯酸12-脂氧合酶(arachidonate 12-lipoxygenase,ALOX12)在结肠癌患者癌组织中的表达及意义。方法:回顾性收集257例结肠癌患者肿瘤及瘤旁组织,免疫组化法检测ALOX12的表达,比较肿瘤及瘤旁组织内表达差异并分析其表达与患者临床病理特征的相关性。随访并探讨ALOX12表达与患者5年总生存率的关系,并采用Kaplan-Meier法绘制生存曲线。运用单因素及多因素Cox回归综合分析影响结肠癌患者5年总生存率的因素。结果:与瘤旁组织相比,ALOX12在肿瘤中阳性表达率更高(63.8%vs.33.1%),差异具有统计学意义(P<0.001)。ALOX12表达与患者年龄、性别、肿瘤部位均无明显相关性(P>0.05),而与TNM分期、淋巴结转移有显著相关性(P<0.05)。ALOX12高表达患者的5年总生存率明显低于低表达患者(P<0.001)。此外,单因素和多因素Cox回归分析结果显示年龄、TNM分期、淋巴结转移、ALOX12表达是影响患者5年总生存率的独立影响因素(P<0.05)。结论:ALOX12高表达与结肠癌TNM分期、淋巴结转移相关,可作为预测患者病情及预后的潜在指标。

Objective:To explore the expression and significance of arachidonate 12-lipoxygenase(ALOX12)in cancer tssues of colon cancer patients.Methods:Tumor and peritumoral tissues of 257 colon cancer patients were retrospectively collected,and the expression of ALOX12 was detected by immunohistochemistry(IHC)to compare the differences in expression within the tumor and peritumoral tissues,and the relationship between ALOX12 expression and clinicopathological features of colon cancer was analyzed.The 5-year overall survival(OS)of the patients was analyzed using Kaplan-Meier curves,and the factors affecting the 5-year OS of the patients were investigated by univariate and multivariate Cox regression analyses.Results:ALOX12 was more positively expressed in tumors compared to peritumoral tissues(63.8%us.33.1%),and the dfference was statistically significant(P<0.001).The expression of ALOX12 was not significantly correlated with the patient's age,gender,and tumor site(P>0.05),but was strongly correlated with TNM staging and lymph node metastasis(P<0.05).Five-year OS of patients with high expression of ALOX12 was markedly lower than those patients with low expression of ALOX12(P<0.001).Single variable and multivariate Cox analyses indicated that age,TNM staging,lymph node metastasis,and ALOX12 expression were independent factors influencing the 5-year OS rate(P<0.05).Conclusion:High expression of ALOX12 is associated with TNM staging and lymph node metastasis of colon cancer,which can serve as a biomarker to predict the patient's condition and prognosis.