流式分选仪的创新调试及在初始T细胞纯化中的应用

Creative Adjustment of the Flow Cell Sorter and the Application in the Purification of Naive T Cells

针对敏感细胞样本如何调试流式分选仪以获得高纯度、高活性的分选产物是众多流式分选从业者关注的问题。该文以流式分选仪FACSAria Fusion为例,首创性地将工程师调试界面与用户调试界面相结合调整系统默认最低样品进样压力,并配合85μm喷嘴,按照用户常规操作流程,在流速1.0的情况下对小鼠原代初始T细胞进行纯化,并对产物的质量进行检测。结果证明阐述的调试方法可以成功地获得高纯度初始T细胞,并且诱导分化成的调节T细胞产生特异转录因子Foxp3的能力与未分选细胞相当。

How to adjust the flow cell sorter for the sensitive cells is a concern of many practitioners.Using the FACSAria Fusion as an example,the article creatively combined the service interface with the user interface to adjust the lowest default sample pressure and then sorted the primary naive T cells according to the standard user’s manual with the 85μm nozzle.The testing quality of the purified naive T cells revealed that the procedure could lead to high purity products.Moreover,treg cells differentiated from the products could generate Foxp3 normally compared with the non-sorting control.