藻毒素降解酶A与微囊藻毒素作用条件的分析

Study on the Interaction Conditions between Microcystinase A and Microcystins

化学生物学的一个重要内容是以生物体中的重要调控酶为靶酶,通过异源表达获得靶酶并研究其性质,实现靶酶的实际应用。以藻毒素降解酶A(MlrA)为中心,成功制备并表征目的蛋白,分析其纯度并探讨不同条件(底物浓度、温度、pH、金属离子)对MlrA降解微囊藻毒素(MCs)活性的影响。通过实验,学生掌握制备和表征目的蛋白的方法,掌握探究不同条件对酶活性影响的实验设计,熟悉重要仪器的使用,进而提高分析问题、解决问题的能力,将理论与实践相结合。在实验教学过程中引导学生思考科学研究对社会的价值和意义,体会科学道德和社会责任的重要性,认识作为科学研究者的社会责任和使命,进而培养并提升学生的科学探究意识和社会责任感。

Targeting regulatory enzymes is an important aspect of chemical biology.The approach involves acquiring target enzymes through heterologous expression,studying their properties,and ultimately applying them in practical settings.Microcystinase A(MlrA)was identified and successfully prepared and characterized as a target enzyme.The study analyzed both the purity of the enzyme and its resulting effects,examining how different substrate concentrations,temperature,pH,and metal ions affected MlrA’s activity in microcystins(MCs)degradation.Through experimentation and exploration,students can learn to prepare and analyze target proteins,design experiments to explore enzyme activity under different conditions,and use key instruments,which improves their problem-solving skills,their ability to apply chemical biology knowledge,and their scientific inquiry and social responsibility.In the process of experimental teaching,students were guided to think about the value and significance of scientific research to society,realize the importance of scientific ethics and social responsibility,and understand the social responsibility and mission of scientific researchers,which cunltivate their senses of scientific inquiry and social responsibility.