香蕉HOS15基因的鉴定及枯萎病菌胁迫下的表达分析

Identification of Banana HOS15 Gene and Expression Analysis Under Stress of Fusarium Wilt

为了研究HOS15(HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 15)是否参与香蕉抗性调控过程,利用拟南芥HOS15蛋白序列,采用多种生物信息学分析工具,对香蕉HOS15(MaHOS15)基因进行鉴定;以高抗品种‘南天黄’和易感品种‘威廉斯’为试验对象,研究MaHOS15在枯萎病菌胁迫下的表达分析及其调控植物病害的潜在机制。结果表明:MaHOS15蛋白中LisH结构域和WD40重复蛋白序列高度保守;MaHOS15蛋白二级结构具有α螺旋、β折叠、延伸链和无规卷曲结构,且以无规卷曲为主,占比为46.2%;亚细胞定位发现MaHOS15在细胞核内,推测MaHOS15可能在细胞核内参与香蕉枯萎病的调控;MaHOS15可能响应SA信号通路、MeJA信号通路和脱落酸反应;推测MaHOS15是在植物抗病过程中负调控植物对病原体防御的关键基因。

In order to study whether HOS15(HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 15)is involved in the regulation of banana resistance process,the Arabidopsis HOS15 protein sequence is used to identify banana HOS15(MaHOS15)gene using various bioinformatics analysis tools,and using the highly resistant variety'Nantianhuang'and the susceptible variety'Williams'as experimental objects,the expression analysis of MaHOS15 under the stress of fusarium wilt and its potential mechanism of regulating plant diseases are studied.The results show that the LisH domain and WD40 repeat protein sequence in MaHOS15 protein are highly conserved.The secondary structure of MaHOS15 protein hasα-helix,β-fold,extended chain and random coil structure,and the random coil is the main structure accounting for 46.2%.Subcellular localization is in the nucleus,which can be supposed that MaHOS15 may be involved in the regulation of banana fusarium wilt in the nucleus.MaHOS15 may respond to SA signaling pathway,MeJA signaling pathway and abscisic acid reaction.It is speculated that MaHOS15 is a key gene in the negative regulation of plant defense against pathogens in the process of plant disease resistance.