基于双金属材料的电化学适体传感器用于磺胺二甲氧嘧啶检测

Electrochemical Aptasensor Based on Bimetallic Composites for Detection of Sulfadimethoxine

开发能够精准检测磺胺二甲氧嘧啶(SDM)的方法在食品安全领域至关重要。该研究利用具有优异电化学活性的镍锌双金属微花(Ni-Zn MFs)作为生物传感平台,同时,引入金锰纳米粒子(AuMn NPs)修饰的还原氧化石墨烯(rGO)作为电信号抑制单元(AuMn/rGO,AMG)。在此基础上,设计了一种用于测定SDM的高灵敏生物传感器。当目标物SDM存在时,能与SDM的结合适配体(Apt)标记的AMG(AMG-Apt)特异性结合,将电信号抑制单元带离电极表面,放大氧化还原探针[Fe(CN)_(6)]^(3-/4-)的电化学信号变化。提出的用于检测SDM的适配体传感器在1 pg/mL~100 ng/mL范围内表现出良好的线性关系,检出限(LOD)低至0.26 pg/mL。此外,该生物传感器在牛奶样品中也表现出令人满意的结果,有望在食品安全分析中应用。

The development of detection techniques for the accurate determination of sulfadimethoxine(SDM)is crucial in food safety.In this study,nickel-zinc bimetallic microflowers(Ni-Zn MFs)with excellent electrochemical activity were utilized as an electrochemical sensing platform,while reduced graphene oxide(rGO)modified with Aurum-Manganese nanoparticles(AuMn NPs)was introduced as an electrical signal hindering molecule(AuMn/rGO,AMG).On this basis,a highly sensitive biosensor for the determination of SDM was developed.When SDM is present,it can specifically bind to the binding Aptamer(Apt)-labeled AMG(AMG-Apt),which carries the electrical signal hindering molecules away from the electrode surface and amplifies the electrochemical signal of the redox probe[Fe(CN)_(6)]^(3-/4-).The proposed aptasensor for the detection of SDM showed good linearity in the range of 1 pg/mL-100 ng/mL with a low limit of detection(LOD)of 0.26 pg/mL.Notably,this biosensor also showed satisfactory results in milk samples,which is expected to be applied in food safety analysis.