术前血清中NPM1和PRDX6的表达对结肠癌根治术后预后的评估价值

The Prognostic Value of the Expressions of NPM1 and PRDX6 in Preoperative Serum After Radical Resection of Colon Cancer

目的探讨术前血清中NPM1和PRDX6的表达对结肠癌根治术后预后的评估价值。方法选取2017年9月至2018年10月入上海市静安区闸北中心医院行结肠癌根治术患者162例,为结肠癌组,同时选取同一段时间在本院体检健康的体检者100例为健康组,qRT-PCR检测NPM1和PRDX6 mRNA表达水平,Pearson相关性分析血清中NPM1和PRDX6表达的相关性,绘制Kaplan-Meier生存曲线,采用Log-Rank检验比较NPM1和PRDX6高低表达者的生存时间。结果结肠癌组患者血清中NPM1和PRDX6 mRNA的相对表达均明显高于健康组(P<0.05);不同的TNM分期、是否淋巴结转移、不同的分化程度、不同肿瘤浸润深度的结肠癌患者血清中NPM1和PRDX6 mRNA表达水平对比差异均有统计学意义(P均<0.05);NPM1与PRDX6表达呈正相关(r=0.534,P<0.001);NPM1高表达患者的生存率明显低于NPM1低表达患者的生存率(P<0.05),PRDX6高表达患者的生存率明显低于PRDX6低表达患者的生存率(P<0.05),Cox单因素分析显示,TNM分期、淋巴结转移、分化程度、肿瘤浸润深度、NPM1和PRDX6是影响结肠癌患者预后的危险因素(P<0.05);Cox多因素分析结果显示,TNM分期、淋巴结转移、分化程度、NPM1和PRDX6是影响结肠癌患者预后的危险因素(P<0.05)。结论NPM1和PRDX6在结肠癌患者血清中高表达,两者的表达与TNM分期、淋巴结转移、分化程度、肿瘤浸润深度及预后有关,可能成为结肠癌预后判断的分子标志物。

Objective To investigate the prognostic value of the expressions of NPM1 and PRDX6 in preoperative serum for colon cancer after radical resection.Methods 162 patients who underwent radical colon cancer surgery at Zhabei Central Hospital in Jing’an District,Shanghai from September 2017 to October 2018 were selected as the colon cancer group,while 100 healthy physical examinees in our hospital during the same period were selected as the healthy group.The expression levels of NPM1 and PRDX6 mRNA were detected by qRT-PCR.Person correlation analysis was applied to analyze the correlation between NPM1 and PRDX6 expressions in serum,and Kaplan Meier survival curve was drawn.Log rank test was used to compare the survival time of those with high and low expressions of NPM1 and PRDX6.Results The relative expressions of NPM1 and PRDX6 mRNA in serum of patients with colon cancer were significantly higher than those of healthy controls(P<0.05);There were significant differences in the levels of NPM1 and PRDX6 mRNA in the serum of colon cancer patients with different TNM stages,lymph node metastasis,differentiation and tumor invasion depth(P<0.05);NPM1 and PRDX6 were positively correlated(r=0.534,P<0.001);The survival rate of patients with high expression of NPM1 was significantly lower than that of patients with low expression of NPM1(P<0.05);Cox multivariate analysis showed that TNM stage,lymph node metastasis,degree of differentiation,NPM1 and PRDX6 were the risk factors affecting the prognosis of patients with colon cancer(P<0.05).Conclusion NPM1 and PRDX6 are highly expressed in the serum of patients with colon cancer.The expressions of NPM1 and PRDX6 are related to TNM stage,lymph node metastasis,degree of differentiation,depth of tumor invasion and prognosis,and could serve as a molecular marker to facilitate the prognosis of colon cancer.