基于文献挖掘的雷公藤制剂建立卵巢功能障碍动物模型分析

Analysis of Establishing Animal Model of Ovarian Dysfunction with Tripterygium Wilfordii Preparation Based on Literature Mining

目的研究近20年公开发表的雷公藤制剂建立卵巢功能障碍动物模型的相关文献,为未来应用雷公藤制剂建立有关卵巢功能障碍动物模型标准化提供数据参考。方法在中国生物医学文献数据库、维普期刊中文服务平台、万方数据知识服务平台、中国知网及Springer、Embase、Web of Science、Cochrane Library、PubMed数据库进行检索,获取2000—2023年公开发表的与雷公藤制剂建立卵巢功能障碍动物模型主题相关的文献,经人工仔细阅读筛选,运用Excel 2021软件将模型类别、实验动物种类、初始年龄、正式实验前动物的筛选标准、给药剂量、给药周期、成模标准及检测指标等数据录入并建立数据库进行统计分析。结果共纳入278篇文献,其中中文文献270篇、英文文献8篇。纳入文献中应用雷公藤制剂建立的卵巢损伤模型较多的分别是:卵巢早衰(POF;96次,34.53%)、卵巢储备功能低下(DOR;80次,28.78%)、早发性卵巢功能不全(POI;27次,9.71%)等;实验动物多选用Sprague Dawley(SD)大鼠(205次,73.74%)、Wistar大鼠(22次,7.91%),昆明小鼠(21次,7.55%);多数实验开始前对实验动物行阴道脱落细胞检测动物的动情周期;造模药物多数采用雷公藤多苷,给药剂量常见50 mg·kg^(-1)(121次,43.53%)、75 mg·kg^(-1)(47次,16.91%)、40 mg·kg^(-1)(41次,14.75%);模型成功多以动情周期紊乱为标准;涉及的检测指标频次最高的是血清性激素(229次,25.87%)。结论现有的雷公藤制剂建立卵巢损伤动物模型,多选用7~12周龄且动情周期规律的成熟雌性SD大鼠。模型评价指标建议综合动情周期、血清性激素、卵巢和子宫脏器指数、卵巢组织病理等结果。但目前仍缺乏对不同造模方式的比较以及造模所用具体参数的确立,有待进一步探究。

To study the published literature on establishing animal models of ovarian dysfunction with tripterygium wilfordii preparation for past 20 years,and to provide data reference for establishing animal models of ovarian dysfunction with tripterygium wilfordii preparation in the future.Methods A search in Chinese Biomedical Literature Database,VIP Journal Chinese Service Platform,Wanfang Data Knowledge Service Platform,Chinese Knowledge Infrastructure,Springer,Embase,Web of Science,Cochrane Library and PubMed databases were conducted,and the published literature related to the topic of the article from 2000 to 2023 was obtained.After careful manual reading and screening,the data including model type,experimental animal type,initial age,screening standard of animals before formal experiment,dosage,administration period,modeling standard and detection index were entered into the database for statistical analysis using Excel 2021 software.Results A total of 278 articles were included,including 270 Chinese articles and 8 English articles.The most common ovarian dysfunction models established by tripterygium wilfordii preparation in the included literature are:premature ovarian failure(POF,96 times,34.53%),ovarian function reserve decline(DOR,80 times,28.78%),and premature ovarian insufficiency(POI,27 times,9.71%).Sprague Dawley(SD)rats(205 times,73.74%),Wistar rats(22 times,7.91%)and Kunming mice(21 times,7.55%)were used as experimental animals.The vaginal exfoliated cells of experimental animals were used to detect the estrous cycle of animals before the start of most experiments.Tripterygium wilfordii polyglycoside suspension was used to establish the animal model,and the dosage was 50 mg·kg^(-1)(121 times,43.53%),75 mg·kg^(-1)(47 times,16.91%)and 40 mg·kg^(-1)(41 times,14.75%).The successfully modeled animals often appeared the disorder of estrous cycle.The detected indicators of high frequency is serum sex hormone(229 times,25.87%).Conclusion Mature female SD rats aged 7-12 weeks with regular estrous cycle are often used to establish the existing animal models of ovarian dysfunction induced by tripterygium wilfordii preparation.The evaluation indexes of the model suggest that estrous cycle,serum sex hormones,ovarian and uterine organ indexes and ovarian histopathology should be integrated.However,there is still a lack of comparison of different modeling methods and the establishment of specific parameters used in modeling,which needs further exploration.