鸢尾素和鸢尾素-纳米脂质颗粒在非小细胞肺癌中的抗癌作用及其机制

The anticancer effects and mechanism of Irisin and Irisin-liposomes in non-small cell lung cancer

目的:探讨鸢尾素和鸢尾素-纳米脂质颗粒(liposomes, LPs)对人类非小细胞肺癌细胞H1299的影响及其潜在作用机制。方法:透射电子显微镜观察纳米颗粒表征和细胞线粒体形态。CCK8和流式细胞术分析鸢尾素和鸢尾素-LPs对H1299细胞活力、凋亡和周期的影响。qRT-PCR和Western blot分析鸢尾素对Nrf2/HO-1信号的影响。结果:鸢尾素和鸢尾素-LPs能显著抑制H1299细胞的生长和细胞周期并促进其凋亡,其中鸢尾素-LPs的抑制效果更为显著。通过细胞骨架着色和线粒体膜电位检测,发现鸢尾素和鸢尾素-LPs能显著改变细胞骨架结构和线粒体功能。此外,鸢尾素和鸢尾素-LPs能显著诱导铁依赖性的脂质过氧化,即铁死亡。最后,鸢尾素和鸢尾素-LPs能显著调整Nrf2/HO-1信号通路的表达。结论:本研究揭示了鸢尾素和鸢尾素-LPs的抗肺癌活性和可能的作用机制,为鸢尾素的临床应用提供了新的理论依据和实验依据。

Objective:To investigate the effects of Irisin and Irisin-liposomes(LPs)on human non-small cell lung cancer H1299 cells and their potential mechanisms of action.Methods:Nanoparticle characterization and cellular mitochondrial morphology were observed by transmission electron microscopy.CCK8 and flow cytometry were used to analyze the effects of Irisin and Irisin-LPs on cell viability,apoptosis,and cycling of H1299 cells.qRT-PCR and Western blot were used to analyze the effects of Irisin on Nrf2/HO-1 signaling.Results:Irisin and Irisin-LPs significantly inhibited the growth and cell cycle of H1299 cells and promoted their apoptosis,with Irisin-LPs having a more pronounced effect.Irisin and Irisin-LPs significantly altered cytoskeletal structures and mitochondrial functions as revealed by cytoskeleton staining and mitochondrial membrane potential detection.Furthermore,Irisin and Irisin-LPs significantly induced iron-dependent lipid peroxidation,i.e.,ferroptosis.In addition,Irisin and Irisin-LPs significantly regulated the expression of the Nrf2/HO-1 signaling pathway.Conclusion:This study reveals the anti-lung cancer activity and potential mechanisms of Irisin and Irisin-LPs,providing new theoretical and experimental evidence for the clinical application of Irisin.