着丝粒蛋白A在宫颈癌组织中的表达及临床意义

Analysis of CENP-A expression and its clinical significance in cervical cancer

目的:分析着丝粒蛋白A(centromere protein A,CENP-A)在宫颈癌中的表达情况,与临床病理参数相关性及临床意义。方法:GEPIA和HPA数据库分析CENP-A基因及蛋白在宫颈癌组织中的表达情况;免疫组化检测宫颈癌中CENP-A蛋白的表达水平,分析其与临床病理参数之间的关系;使用Linked Omics数据库寻找与CENP-A共表达基因,分析CENP-A可能参与宫颈癌发生发展的信号通路。结果:生物信息学分析显示宫颈癌组织中CENP-A基因和蛋白的表达水平明显高于癌旁组织(P<0.05);免疫组化结果显示宫颈癌组织CENP-A蛋白表达水平显著高于癌旁组织(P<0.05),且与患者的肿瘤分期及浸润程度相关。通过基因共表达分析,KIF2C、CDC20、FAM72B等基因与CENP-A表达呈正相关,PARM1、ABCA9和SYNE1等基因与CENP-A表达呈负相关(FDR<0.05),CENP-A基因参与了细胞周期、DNA复制等途径。结论:CENP-A在宫颈癌组织中高表达,与宫颈癌的预后以及发生发展相关。

Objective:To analyze the expression of centromere protein A(CENP-A)in cervical cancer,its correlation with clinicopathological parameters and its clinical significance.Methods:Gene Expression Profiling Interactive Analysis(GEPIA)database and Human Protein Atlas(HPA)database were used to analyze the expression of CENP-A gene and protein in cervical cancer tissues and adjacent tissues.The expression level of CENP-A protein in cervical cancer tissues and adjacent tissues was detected by immunohistochemistry,and the relationship between CENP-A protein expression and clinicopathological parameters was analyzed.Using the Linked Omics database to explore the co-expressed genes of CENP-A,and further analyze the signal regulatory pathways that CENP-A gene may participate in the development of cervical cancer.Results:The bioinformatics analysis showed that the expression level of CENP-A in cervical cancer tissues was significantly higher than that in the adjacent tissues(P<0.05),and the expression of CENP-A protein in cervical cancer tissues is higher than that in the adjacent tissues.The results of immunohistochemistry showed that the expression level of CENP-A protein in cervical cancer tissues was higher than that in the adjacent tissues(P<0.05),and the expression of CENP-A was correlated with tumor stage and degree of invasion.Through gene co-expression analysis,KIF2C,CDC20,FAM72B and other genes were positively correlated with CENP-A expression,while PARM1,ABCA9 and SYNE1 were negatively correlated with CENP-A expression(FDR<0.05).CENP-A gene is involved in cell cycle,DNA replication and other pathways.Conclusion:CENP-A is highly expressed in cervical cancer tissues,which is related to the prognosis,occurrence and development of cervical cancer.