M2型丙酮酸激酶、缺氧诱导因子-1α在子宫内膜癌组织中的表达及与患者临床特征的关系

Expression of pyruvate kinase M2,hypoxia-inducible factor-1αin endometrial cancer tissues and their relationship with clinical characteristics of patients

目的探讨M2型丙酮酸激酶(PKM2)、缺氧诱导因子-1α(HIF-1α)在子宫内膜癌(EC)组织中的表达及与患者临床特征的关系。方法取41例EC患者的EC组织及相应癌旁组织和10例子宫内膜良性疾病患者的正常子宫内膜组织。采用免疫组化法检测PKM2、HIF-1α的表达情况。比较EC组织、癌旁组织及正常子宫内膜组织中PKM2、HIF-1α的阳性表达率及不同临床特征EC患者EC组织中PKM2、HIF-1α的表达情况。结果EC组织中HIF-1α的阳性表达率明显高于癌旁组织和正常子宫内膜组织,差异均有统计学意义(P﹤0.01)。p53突变型、分化程度为低分化EC患者EC组织中PKM2的阳性表达率分别明显高于p53野生型、分化程度为中高分化患者,差异均有统计学意义(P﹤0.01)。有脉管浸润的EC患者EC组织中HIF-1α的阳性表达率高于无脉管浸润患者,差异有统计学意义(P﹤0.05)。结论HIF-1α在EC组织中的阳性表达率较高。PKM2、HIF-1α表达与EC患者的临床特征有关,或可作为EC患者的有效治疗靶点。

Objective To investigate the expression of pyruvate kinase M2(PKM2),hypoxia-inducible factor-1α(HIF-1α)in endometrial cancer(EC)tissues and their relationship with clinical characteristics of patients.Method The EC tissues and adjacent paracancerous tissues from 41 patients with EC,and the normal endometrial tissues from 10 patients with benign endometrial diseases were selected.The expression of PKM2 and HIF-1αwere detected by immunohistochemical method.The positive expression rates of PKM2 and HIF-1αin EC tissues,paracancerous tissues and normal endometrial tissues were compared,and the expression of PKM2 and HIF-1αin EC tissues of EC patients with different clinical characteristics were compared.Result The positive expression rate of HIF-1αin EC tissues was significantly higher than that in paracancerous tissues and normal endometrial tissues,and the differences were statistically significant(P<0.01).The positive expression rates of PKM2 in EC tissues of EC patients with p53-mutant and poorly differentiated were significantly higher than those in patients with p53-wild,medium and highly differentiated,and the differences were statistically significant(P<0.01).The positive expression rate of HIF-1αin EC tissues of EC patients with vascular invasion was higher than that in patients without vascular invasion,and the difference was statistically significant(P<0.05).Conclusion The positive expression rate of HIF-1αis high in EC tissues.The expression of PKM2 and HIF-1αare related to the clinical characteristics of EC patients,which may be effective therapeutic targets for EC patients.