miR-934和miR-1307在食管鳞癌患者血清中的表达水平及与临床病理特征和预后的关系

Expression levels of miR-934 and miR-1307 in serum of patients with esophageal squamous cell carcinoma and their relationship with clinicopathological features and prognosis

目的明确miR-934和miR-1307在食管鳞癌(ESCC)患者血清中的表达水平,并分析其表达与患者临床病理特征和预后的关系。方法选取2018年1月至2019年12月本院收治的ESCC患者125例(ESCC组),另选取同期体检健康者100例(对照组),利用实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测血清中miR-934和miR-1307的表达。随访3年,以二者均值将患者分为miR-934低表达组(<2.53)和miR-934高表达组(≥2.53),miR-1307低表达组(<2.10)和miR-1307高表达组(≥2.10),比较血清中miR-934和miR-1307不同表达患者的预后情况,并通过单因素和多因素COX回归分析影响患者生存预后的相关因素。结果ESCC组患者血清miR-934和miR-1307的相对表达量分别为2.53±0.72和2.10±0.68,明显高于对照组的1.32±0.39和1.12±0.33(均P<0.05)。血清miR-934和miR-1307与ESCC患者TNM分期、淋巴结转移、肿瘤分化程度以及远处转移密切相关(均P<0.05)。血清miR-934和miR-1307高表达组3年无进展生存期(PFS)低于miR-934和miR-1307低表达组。多因素Cox回归分析结果显示,TNM分期Ⅲ~Ⅳ期、miR-934高表达和miR-1307高表达、肿瘤中低分化程度、远处转移是影响ESCC患者PFS的独立危险因素(均P<0.05)。结论血清miR-934和miR-1307在ESCC患者中呈异常高表达,且其高表达是ESCC患者不良预后的独立危险因素,有望成为ESCC的预后评估的标志物。

Objective To clarify the expression levels of miR-934 and miR-1307 in serum of patients with esophageal squamous cell carcinoma(ESCC),and analyze the relationship between their expression and clinicopathological features and prognosis.Methods A total of 125 ESCC patients(ESCC group)who were admitted to our hospital from January 2018 to December 2019 were selected,and 100 healthy subjects(control group)were selected during the same period,the expression of miR-934 and miR-1307 in serum were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR).After 3 years of follow-up,the patients were divided into miR-934 low expression group(<2.53)and miR-934 high expression group(≥2.53),miR-1307 low expression group(<2.10)and miR-1307 high expression group(≥2.10),based on their mean values,the prognosis of patients with different expression of miR-934 and miR-1307 in serum was compared,and the related factors affecting the survival and prognosis of patients were analyzed by univariate and multivariate COX regression analysis.Results The relative expression levels of serum miR-934 and miR-1307 in ESCC group were 2.53±0.72 and 2.10±0.68 respectively,significantly higher than those in control group′s 1.32±0.39 and 1.12±0.33(all P<0.05).Serum miR-934 and miR-1307 were closely related to TNM stage,lymph node metastasis,tumor differentiation and distant metastasis in ESCC patients(all P<0.05).The 3-year progression-free survival(PFS)in serum miR-934 and miR-1307 high expression group was lower than that in miR-934 and miR-1307 low expression group.Multivariate Cox regression analysis showed that,TNM stage Ⅲ~Ⅳ,high expression of miR-934 and high expression of miR-1307,low differentiation degree and distant metastasis were independent risk factors for PFS in ESCC patients(all P<0.05).Conclusion Serum miR-934 and miR-1307 are abnormally highly express in ESCC patients,and their high expression is an independent risk factor for poor prognosis of ESCC patients,which is expected to be a marker for prognosis evaluation of ESCC.