基于网络药理学探讨蒙药森登-9汤治疗布鲁氏菌病的作用机制

Mechanism of Mongolian medicine Sendeng-9 decoction in the treatment of brucellosis based on network pharmacology based on network pharmacology

目的:运用网络药理学的方法,探讨蒙药森登-9汤(布病Ⅱ号)治疗布鲁氏菌病的作用机制。方法:运用BATMAN-TCM数据库分析蒙药森登-9汤的活性成分及潜在靶点;通过Genecards、OMIM、DisGeNET数据库筛选布鲁氏菌病相关靶点;构建韦恩图,得到疾病交集靶点与蒙药森登-9汤治疗布鲁氏菌病的交集靶点;用Cytoscape软件绘制“药物-成分-疾病-靶点”关系网络图;通过STRING数据库构建“活性成分-疾病”共同靶点的蛋白互作(PPI)网络;使用David数据库对关键靶标进行基因本体功能(GO)及京都基因和基因组百科全书(KEGG)通路富集分析。结果:蒙药森登-9汤活性成分有182个,作用于1306个靶点;布鲁氏菌病靶点620个,药物与疾病交集靶点152个。蒙药森登-9汤治疗布鲁氏菌病的主要活性成分有月桂酸、肉豆蔻酸、亚油酸、茉莉酮、羽扇烯酮、香叶基丙酮、氧化槐果碱、3-壬烯-2-酮等;治疗布鲁氏菌病的核心靶点有IL-6、IL-1β、INS、TNF、PTGS2、JUN、TP53、IGF1、CCL2、HIF1A。富集得到GO条目1064个,其中BP条目834个,CC条目107个,MF条目123个。KEGG信号通路共162条,主要集中在HIF-1信号通路、TNF信号通路、MAPK信号通路、FoxO信号通路、IL-17信号通路等。结论:本研究通过网络药理学的方法初步探讨了蒙药森登-9汤有效成分抗炎、抗病原微生物等作用机制,为布鲁氏菌病的治疗,及进一步研究该方作用机理、临床应用提供了理论依据。

Objective:To explore the mechanism of Mongolian medicine Sendeng-9 Decoction(Brucellosis Ⅱ)in the treatment of brucellosis by network pharmacology.Methods:The BATMAN-TCM database was used to analyze the active components and potential targets of Mongolian medicine Sendeng-9 decoction.Brucellosis-related targets were screened through Genecards,OMIM,and DisGeNET databases.The Venn diagram was constructed to obtain the intersection target of disease and the intersection target of Mongolian medicine Sendeng-9 decoction in the treatment of brucellosis.The network diagram of“drug-component-disease-target”relationship was drawn by Cytoscape software.The protein-protein interaction(PPI)network of'active ingredient-disease'common targets was constructed by STRING database.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of key targets were performed using the David database.Results:There were 182 active components of Mongolian medicine Sendeng-9 Decoction,which acted on 1306 targets.There were 620 brucellosis targets and 152 drug-disease intersection targets.The main active ingredients of Mongolian medicine Sendeng-9 Decoction in the treatment of brucellosis were lauric acid,myristic acid,linoleic acid,jasmonone,lupinone,geranylacetone,oxysophocarpine,3-nonen-2-one,etc.The core targets for the treatment of brucellosis were IL-6,IL-1β,INS,TNF,PTGS2,JUN,TP53,IGF1,CCL2,HIF1A.A total of 1064 GO entries were obtained,including 834 BP entries,107 CC entries,and 123 MF entries.There were 162 KEGG signaling pathways,mainly focusing on HIF-1 signaling pathway,TNF signaling pathway,MAPK signaling pathway,FoxO signaling pathway,IL-17 signaling pathway and so on.Conclusion:Through the method of network pharmacology,this study preliminarily discusses the anti-inflammatory and disease-resistant pathogenic microorganisms of the effective components of Mongolian medicine Senden-9 decoction,which provides a theoretical basis for the treatment of brucellosis,and further study of the mechanism of action and clinical application of this prescription.