亮菌多糖体外模拟消化-酵解特性及其益生作用探究

Study on the simulated digestion-fermentation characteristic and probiotic effect of polysaccharide from Armillariella tabescens in vitro

天然多糖活性功能丰富,为了进一步探究真菌多糖活性作用方式与途径,确定亮菌多糖益生潜能,该研究用液态发酵法提取并制备亮菌胞外多糖(Armillariella tabescens extracellular polysaccharide,ATEP),去除大分子杂质后评价多糖制备得率,分析理化组成,通过体外模拟消化和模拟酵解,分阶段评价ATEP消化后抗氧化能力,及消化过程中的变化规律。结果表明,ATEP总糖占比43.52%,ATEP两组分分子质量分别为27.0 kDa和8.9 kDa,多糖由6种单糖构成;模拟消化过程几乎不会对总糖及还原糖含量造成变化(P>0.05),但可提高总酚及总黄酮含量(P<0.05);模拟酵解过程碳水化合物相对消耗量逐步增加,而还原糖先增再少,pH值则持续下降;酵解产物SCFAs含量增加显著(P<0.05),其中乙酸含量增量最多;ATEP可提高双歧杆菌、植物乳杆菌、干酪乳杆菌及大肠杆菌4种益生菌增殖速度。本研究评价了ATEP的益生水平,为综合利用亮菌多糖提供思路与基础理论依据。

Natural polysaccharides have rich active functions.In order to further explore the pathway of activity and to determine the probiotic potential of Armillariella tabescens polysaccharides,this study prepared Armillariella tabescens extracellular polysaccharide(ATEP)by liquid fermentation,and evaluated the physicochemical compositions,the antioxidant capacity and the changes rule during different stages of the simulated digestion-fermentation process.The results showed that the total sugar content of ATEP was 43.52%,the molecular weights of the two components of ATEP were 27.0 kDa and 8.9 kDa,respectively.ATEP was composed of six monosaccharides.The simulated digestion process hardly promoted the total sugar and reducing sugar contents(P>0.05),but could increase the total phenol and total flavonoid contents(P<0.05).During the simulated fermentation process,the relative consumption of carbohydrates gradually rose,reducing sugar falling down after growing,while pH continued to decrease.The contents of SCFAs in fermental production increased significantly(P<0.05),and the acetic acid was the highest.ATEP promoted the proliferation rate of four probiotics,Bifidobacterium,Lactobacillus plantarum,Lactobacillus casei and Escherichia coli.This study evaluates the probiotic level of ATEP,and provides ideas and basic theoretical basis for the comprehensive evaluation and utilization of it.