模拟肠液培养基中壳寡糖对益生菌生长的影响

Effects of Chito-oligosaccharides on the growth of probiotics in simulated intestinal medium

文章探究了模拟肠液培养基中壳寡糖对益生菌生长的影响,为益生菌、壳寡糖合生元的开发提供理论依据。将益生菌接种至含不同浓度壳寡糖的模拟肠液培养基中培养,通过测定菌体密度(△OD_(600))、活菌数的变化,测定△OD_(260)的变化以及用扫描电子显微镜表征细胞膜的完整性,明确模拟肠液培养基中壳寡糖对益生菌生长、细胞膜完整性的影响。结果显示8株专利益生菌都可以在模拟肠液培养基中正常生长,都不能以壳寡糖为唯一碳源生长。自然培养过程模拟肠液培养基中壳寡糖抑制8株专利益生菌的生长;壳寡糖处理导致益生菌细胞膜表面塌陷及褶皱,破坏了益生菌细胞膜的完整性。偏碱性模拟肠液培养基中壳寡糖对8株专利益生菌的生长没有统一的抑制作用。偏碱性模拟肠液环境下,壳寡糖对益生菌生长的抑制作用较弱,或没有抑制作用,可以将益生乳酸菌与壳寡糖复配开发合生元,用于调节肠道微生态。

The objectice of this paper is to explore the effects of Chito-oligosaccharide on the growth of probiotics in stimulated intestinal fluid culture medium,so as to provide a theoretical basis for the development of probiotic and Chito-oligosaccharide synbiotics.Method:Eight probiotics were inoculated into simulated intestinal fluid culture medium containing different concentrations of Chito-oligosaccharide for cultivation.The changes of cell density(△OD_(600)),viable bacteria count,and the absorbance of culture at△OD_(260) nm were measured,as well as the integrity of cell membrane was characterized by scanning electron microscope.Results:all 8 probiotics grew normally in simulated intestinal fluid culture medium,and cannot grow with Chito-oligosaccharide as the sole carbon source.Chito-oligosaccharide inhibited the growth of 8 probiotics in simulation simulated intestinal fluid culture medium under natural cultivation process.Chito-oligosaccharide treatment led to surface collapse and wrinkles of probiotic cell membranes,disrupting the integrity of probiotic cell membranes.However,there was no unified inhibitory effect of Chito-oligosaccharide on the growth of 8 patented probiotics in alkaline simulated intestinal fluid culture medium.Conclusion:under alkaline simulated intestinal fluid environment,Chito-oligosaccharide has a weak or no inhibitory effect on the proliferation of probiotics.Probiotics can be combined with Chito-oligosaccharide to develop synbiotics for regulating intestinal microecology.