CeNPs在DOX诱导的心脏毒性中的作用及对SOD的影响

Effect of CeNPs for DOX Induced Cardiotoxicity and Their Influence on SOD

目的 探究CeNPs(纳米氧化铈)在DOX(阿霉素)诱导的心肌细胞损伤中的作用,以及CeNPs对DOX心肌细胞损伤中SOD活性的影响。方法 实验使用H9C2大鼠心肌细胞,DOX处理建立损伤模型,并将细胞随机分为5组:空白对照组、DOX损伤组、CeNPs组、DOX+CeNPs组、DOX+DEX(右丙亚胺,阳性保护药)组进行实验。通过CCK-8法测定存活率、Western Blot法测定蛋白表达量、生化法检测氧化应激指标SOD活性。结果 CCK-8法:DOX+CeNPs组相对于DOX组,细胞存活率显著增高(P<0.0001)。Western Blot法:DOX+CeNPs组与DOX组对比,Bax蛋白表达水平下降(P<0.0001),Bcl-2蛋白表达水平增高(P<0.05)。生化法检测:DOX+CeNPs组与DOX组对比,SOD活力升高(P<0.05)。结论 CeNPs能够提高DOX处理后的细胞存活率;CeNPs调控相关凋亡蛋白的表达减轻DOX处理后的细胞凋亡;CeNPs通过提高SOD活性改善DOX处理后的细胞氧化应激失平衡。

Objective To investigate effect of CeNPs(nano ceria) for DOX(doxorubicin) induced myocardial cell injury,and influence of CeNPs on SOD activity in DOX induced myocardial cell injury.Methods The experiment established injury model with H9C2 rat cardiomyocytes after DOX treatment,and randomly divided cells into 5 groups:blank control group,DOX injury group,CeNPs group,DOX+CeNPs group,and DOX+DEX(positive protective drug) group for experiment,and measured survival rate by CCK-8 method,protein expression by Western Blot method,and oxidative stress indicator SOD activity by biochemical method.Results CCK-8 method showed cell survival rate in DOX+CeNPs group was significantly higher than DOX group(P<0.0001).Western Blot methods showed,expression level of Bax protein in DOX+CeNPs was lower than DOX group,(P<0.0001),expression level of Bcl-2 protein was higher,(P<0.05).Biochemical detection:DOX+CeNPs group showed higher SOD activity than DOX group,(P<0.05).Conclusion(1) CeNPs can improve cell survival rate after DOX treatment;(2) CeNPs can alleviate cell apoptosis after DOX treatment by regulating expression of apoptosis related proteins;(3) CeNPs can improve cellular oxidative stress imbalance after DOX treatment by increasing SOD activity.